TNF alpha Protein
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- Target Alle TNF alpha Proteine anzeigen
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Ratte
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human Tumor necrosis factor (TNF-alpha) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin-Niveau
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7 units/mg.
- Top Product
- Discover our top product TNF alpha Protein
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handhabung
- Resuspend the protein in the desired concentration in proper buffer
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Andere Bezeichnung
- Tumor Necrosis Factor (Tnf-Alpha) (TNF alpha Produkte)
- Synonyme
- DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
- Molekulargewicht
- 17.3 kDa
- NCBI Accession
- NP_036807
- Pathways
- NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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