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Polyklonale Primärantikörper

Polykolonale Antikörper (pAbs) sind heterogene Mischungen von Immunglobulinen, die von mehreren B-Zellklonen produziert werden und mehrere Epitope eines einzelnen Antigens erkennen. Diese breite Epitoperkennung ermöglicht eine hohe Sensitivität und robuste Signalstärke, wodurch sich polyklonale Antikörper besonders für den Nachweis von niedrig exprimierten Proteinen oder strukturell variablen Targets eignen.

Durch die Bindung an verschiedene Regionen eines Antigens zeigen polyklonale Antikörper eine zuverlässige Performance in Anwendungen, bei denen Epitope teilweise denaturiert oder maskiert sein können, wie z. B. Western Blot oder Immunhistochemie (IHC). Sie werden häufig in ELISA, WB, IHC, IF und Immunpräzipitation eingesetzt, wenn eine starke und konsistente Signalgebung erforderlich ist.

Gleichzeitig führt ihre tierbasierte Herstellung zu einer inhärenten Batch-to-Batch-Variabilität, die die langfristige Reproduzierbarkeit beeinflussen kann. Zudem kann die Bindung an mehrere Epitope zu einer geringeren Spezifität im Vergleich zu monoklonalen oder rekombinanten Antikörpern führen. Daher eignen sich polyklonale Antikörper besonders für explorative Studien, den Nachweis schwach exprimierter Targets oder Anwendungen, bei denen Sensitivität wichtiger ist als maximale Spezifität.

Für Anwendungen, die eine hohe Lot-to-Lot-Konsistenz oder eine präzise Target-Diskriminierung erfordern, sind monoklonale oder rekombinante Antikörper oft besser geeignet. Multiklonale Antikörper bieten eine ausgewogene Alternative, indem sie breite Epitoperkennung mit verbesserter Reproduzierbarkeit kombinieren.

Bei antibodies-online umfasst unser Portfolio polyklonaler Antikörper validierte Antikörper für ELISA, Western Blot, Immunhistochemie, Immunfluoreszenz und Immunpräzipitation. Mit breiter Target-Abdeckung und anwendungsspezifischen Validierungsdaten bieten diese Antikörper zuverlässige Werkzeuge für den sensitiven Proteinnachweis in unterschiedlichsten Forschungsanwendungen.

Wichtige Targets

Alle Polyklonalen Antibodies

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RFP Antikörper

RFP Reaktivität: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 Abbildungen

363 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN129578

350,00 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 1 bis 2 Werktagen

Independently Validated

beta Actin Antikörper (AA 1-50)

ACTB Reaktivität: Human, Maus, Ratte, Kaninchen, Schwein, Rind (Kuh) WB, ELISA, IHC (p), FACS, IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Rat lung lysate 30ug, probed (ABIN724340) at 1:200 overnight in 4 °C.

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Human HeLa cell lysates probed with Rabbit Anti-beta Actin Polyclonal Antibody, Unconjugated .

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Hepatic overexpression of HNF4α in rats decreased cholesterol levels in both plasma and liver.

Expression pattern of hepatic cholesterol-regulatory genes under HNF4α overexpression in rat liver.

HNF4α-upregulated SR-BI depends on PPARγ.

High-temperature cultivation of an rhIL-10 expression strain impairs the maturation of G3-pro-rhIL10.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

Expression of Mx and interferon (IFN) genes in ZF4 with time after SHVV infection.

Expression of Retinoic acid-inducible gene I (RIG-I), Melanoma differentiation-associated gene 5 (MDA5), LGP2T, and LGP2a genes in ZF4 with time after SHVV infection.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Pro-Gly, but not Pro plus Gly (Pro+Gly), promoted the expression and secretion of IGF-1 in the HepG2 cells.

Knockdown of PepT1 eliminated the increase of IGF-1 expression induced by Pro-Gly in the HepG2 cells.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Inhibition of JAK2/STAT5 signaling pathway blocked the promotive effect of Pro-Gly on IGF-1 expression and secretion in the HepG2 cells.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Injection of JAK2/STAT5 inhibitor abolished the promotive effect of Pro-Gly on IGF-1 expression and secretion in mice.

Protein expression of MTA1 by Western Blot analysis.

Effects of stable YAP1 silencing in BGC-823 cells and stable YAP1 overexpressing in GES-1 cells on migration and invasion abilities in vitroThe wound healing assay was used to evaluate the migration properties of BGC-823 GC cells (A, G) and GES-1 cells (B, H).

Effects of JQ1 treatment on cell proliferation, migration and invasion in ccRCC cell lines(A) Cell proliferation was determined by XTT assays (*P < 0.

JQ1 treatment inhibited MYC expression in ccRCC cells(A) The expression level of MYC mRNA was significantly upregulated in ccRCC tissues compared with that in normal kidney tissues according to TCGA data (P < 0.

Protein levels of NF-κB and NLRP3/Caspase-1 pathway in CIA rats.

Clinical significance of BRD4 expression in ccRCC according to TCGA data(A) Overall survival (OS) was significantly shortened in patients with high BRD4 expression (top third) compared with that in patients with medium and low BRD4 expression (P = 0.

The effect of LBP on the expression of GFAP, VEGF, and PEDF in the retinal tissue.

Relative expression of AANAT, HIOMT, MT1, and MT2 in COCs of follicles of different sizes.

Effect of E2 and ER antagonist ICI182780 on melatonin production and the expression of AANAT, HIOMT, MT1, and MT2 mRNA and protein in sheep COCs.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

43 Abbildungen

272 Referenzen

Produktnummer ABIN724340

120,76 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

COL1A2/COL1A1 Antikörper

Rockland 600-401-103-0.1 Reaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte DB, ELISA, FLISA, FACS, FM, IHC, IP, WB Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunofluorescence staining of extracellular and intracellular type I collagen.

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

46 Abbildungen

63 Referenzen

Independent Validation

2 validations

Produktnummer ABIN5596819

640,81 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 5 bis 8 Werktagen

Independently Validated

E-cadherin Antikörper (AA 401-500)

CDH1 Reaktivität: Human, Maus, Ratte WB, FACS, ELISA, ICC, IHC (p), IF (p), IHC (fro), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.

MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

Image provided by One World Lab validation program.

Image provided by Independent Validation badge 29768.

Image provided by One World Lab validation program.

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

14 Abbildungen

14 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN1387847

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

CTNNB1 Antikörper (N-Term)

KO Validated CTNNB1 Reaktivität: Human WB, IHC, IF, FACS, IHC (p), IP, ICC, IHC (fro), ChIP, CUT&RUN, IHC (wm) Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 Abbildungen

11 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2855042

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Independently Validated

SOX2 Antikörper

KO Validated SOX2 Reaktivität: Human WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2, antibody at 1:100 dilution.

IP Image Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] , or SOX2 antibody [GT1876], Western blot analysis was performed using SOX2 antibody [N1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2, antibody at 1:160 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2, antibody at 1:80 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.

FACS Image Flow cytometry on human embryonic stem cells, staining with SOX2 ,antibody at 1:100 dilution(purple) or rabbit IgG (black).

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

Western blot analysis of CD44, octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), SOX9, transketolase (TKT), hypoxia-inducible factor 1 alpha (HIF-α) and phosphoglycerate kinase 1 (PGK-1) in CSC-like and non-CSC UM1 cells (A).

KD of Cops2, but not any other CSN subunits, reduces the expression of Nanog and Oct4.

In vitro characterizations of hc-iPSCs.

S1PR1 activation enhanced NSCs proliferation and neuronal differentiation in hippocampal dentate gyrus (DG) after TBI.

S1PR1-induced NSCs proliferation and neuronal differentiation in TBI rats were affected by MEK/Erk activity.

17 Abbildungen

12 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2855074

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Independently Validated

Iba1 Antikörper (C-Term)

IBA1 Reaktivität: Human WB, IHC, ICC, IF, FACS, IHC (p), IHC (fro), IHC (ff) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 Abbildungen

15 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2857032

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Independently Validated

Caspase 8 Antikörper (AA 411-482)

CASP8 Reaktivität: Human, Maus, Ratte, Affe, Schwein, Schaf WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 Abbildungen

15 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN724205

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

TNF alpha Antikörper (AA 181-235)

TNF alpha Reaktivität: Human, Maus, Ratte, Kaninchen, Pferd WB, ELISA, IHC (p), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Histology and immunofluorescence in rat corneal sections.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 Abbildungen

21 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN677318

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

Dendra 2 Antikörper

Reaktivität: Koralle WB, FACS, ICC Wirt: Kaninchen Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct, reducing and non-reducing conditions.

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

8 Abbildungen

19 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN361314

356,73 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μg

Lieferung nach: Deutschland

Lieferung in 2 bis 4 Werktagen

Independently Validated

CD63 Antikörper (AA 120-175)

CD63 Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh), Hund, Kaninchen, Huhn, Esel, Ziege, Meerschweinchen, Hamster, Pferd, Schwein, Schaf, Cat, Avian WB, IF, IHC (p), IHC (fro) Wirt: Ziege Polyclonal unconjugated

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Anti-CD63 Ab at 1/1,000 dilution; Iysate at 50 µg per lane; Rabbit polyclonal to goat lgG (HRP) at 1/10,000 dilution

Immunofluorescence — anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;

Immuno-localization of CD63 in the hen reproductive trac

Representative immunogold labeling (using TEM) images of CSF-derived EVs from control and prenatally cocaine-exposed male and female monkeys are shown.

Flow cytometry analysis of membrane CD63 level in PMN of control and SCK cows.

Expression of exosomes in bronchoalveolar lavage in Rat BAL fluid Source: PMID36035371

Expression of exosomes in Rat lung tissue Source: PMID36035371

Expression of exosomes in Rat Brain Tissue Source: PMID36035371

Immunofluorescence (IF) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

18 Abbildungen

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Independent Validation

3 validations

Produktnummer ABIN1440014

465,38 €

Zzgl. Versandkosten 20,00 € und MwSt

600 μg (Weitere Mengeneinheiten)

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Lieferung in 2 bis 4 Werktagen

Independently Validated

GFP Antikörper

GFP Reaktivität: Aequorea victoria WB, ELISA, FM Wirt: Ziege Polyclonal unconjugated

Western Blot of Goat anti-GFP antibody.

MSCs are traced in special organized regions.

Immunofluorescence Microscopy of GFP-GOAT-Antibody.

Western Blot of Rabbit anti-GFP antibody.

Immunofluorescence Microscopy of GFP-GOAT-Antibody.

Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody.

Western Blot of Goat anti-GFP antibody with lysates.

Western Blot of Goat anti-GFP Antibody with Serums.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Maximal activity of HIPKs depends on the activation loop tyrosine.

Subcellular distribution of HIPK mutants.

15 Abbildungen

178 Referenzen

Produktnummer ABIN100085

350,00 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL (Weitere Mengeneinheiten)

Lieferung nach: Deutschland

Lieferung in 1 bis 2 Werktagen

GAPDH Antikörper

GAPDH Reaktivität: Human WB, IF, IP, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in mouse testis by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH, antibody (green) at 1:500 dilution.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IP Image Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody, Western blot analysis was performed using GAPDH antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of human salivary gland cancer, using GAPDH, antibody at 1:500 dilution.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in rat heart by immunohistochemical analysis.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Effects of quercetin on the inflammatory response in H2O2-treated H9C2 cells.

Effects of quercetin on cell apoptosis in H2O2-treated H9C2 cells.

Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS.

Gene trap screen for mitochondrial phenotypes identifies Sucla2 mutant allele.

Sucla2 mutant embryos display growth deficiency and placental mtDNA depletion.

Sucla2-deficient embryo tissues exhibit progressive, functionally significant mtDNA depletion and elevated MMA.

CM extract induces cell apoptosis.

Effects of quercetin on doxorubicin-induced changes of cell viability, cell apoptosis and cell morphology in H9C2 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

RAGE expression in human peripheral nerve fibers.

Arecoline induces the transdifferentiation of buccal mucosal fibroblasts (BMFs) into myofibroblasts.

Arecoline-induced α-smooth muscle actin (α-SMA) expression is mediated by the direct binding of zinc finger E-box binding homeobox 1 (ZEB1) to the α-SMA promoter.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Regulation of NED by REST in LNCaP cells.

Regulation of NED by REST in LNCaP cells.

Regulation of NED by REST in LNCaP cells.

IL-6 induces NED in LNCaP cells and this is concomitant with increased autophagy.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

Modulation of curcumin in oxidative stress-regulating genes.

Arecoline decreased membrane expression of ZO-1 in confluent TM4 cells.

Arecoline activates ERK1/2 MAPKs and increases TNF-alpha production.

Activation of ERK1/2 MAPKs is important for TNF-alpha induction and ZO-1 protein redistribution by arecoline in TM4 cells.

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway.

Early and accelerated processing of caspase-8 with DZNep treatment in lymphoma cell lines.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

Effects of different proteins on post-treatment lipolysis markers.

Creation and characterization of Supt4h knockout mice.

Role of STAT3 expressionA.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Dicer inactivation in mutant mice.

The expression of BH3-only protein, pro-apoptotic proteins and anti-apoptotic proteins in LNCaP or PC3 cells after treatment with paclitaxel.

The effect of ABT-199, ABT-263 or ABT-263 in combination with paclitaxel on LNCaP or PC3 cells.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

Diverse fuels promote TNKS autoPARsylation and turnover in INS-1 cells.

Promoter hypermethylation of sGCβ1 in MCF-7 and sGCα1 in MDA-MB-231 cells.

Over-expression of sGCα1 and sGCβ1 in MDA-MB-231 cells affected cGMP production, proliferation, survival, and cell cycle.

Over-expression of sGC induced apoptosis and cell cycle arrest.

HYS-32 modulates GSK3β phosphorylation.

GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes.

Caspase-dependent apoptotic pathways were seen in LNCaP, but not in PC3 cells, in response to G2/M arrest.

LY294002 inhibits the HYS-32-induced phosphorylation of GSK3β-pS9 and GSK3β-pY216.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Effects of TLCA on HDAC4 expression in cultured primary HSCs.

Overexpression of miR-29a decreased HDAC4 expression in primary HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Western blot analysis shows pErk reduction through selumetinib treatment and by removal of stromal support.

Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Mek pathway inhibition in combination-treated BCP-ALL cells by phospho-flow and Western blot.

Demyelination/remyelination and decreased P0 expression in dKO mice.

Loss of neurofascins and Caspr in dKO mice.

P0 interacts with neurofascins.

ETO inhibits ACT cell growth.

Inhibition of ERK1/2 inhibits ETO-induced centrosome amplification.

Chloroquine inhibits DNA damage-induced centrosome amplification.

Chloroquine inhibits ETO-activated CDK2 and ERK1/2 in adrenocortical Y1 tumor cells.

HSPs positively regulate IE2 transaction activity.

Comparison of the mRNA levels of Smad7 (a) and protein levels of Smad3 (b) expressions in the liver of WT and miR-29Tg mice following bile duct ligation (BDL).

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

SFN suppressed HCV protein expression and RNA replication.

SFN-mediated HO-1 induction in HCV replicon cells.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN induced bilirubin production in HCV replicon cells.

SFN inhibited HCV replication by upregulating Nrf2 expression.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days.

Inhibition of neurite elongation by REST overexpression(A) LNCaP-TR-REST cells were treated with 0.

AMPK/mTOR pathway is activated by hypoxia treatment and REST knockdown(A) LNCaP cells were treated with normoxia (N) or hypoxia (H) (2 % O2) for 3 days (left panel).

Hypoxia-induced autophagy activation of LNCaP cells(A) LNCaP-eGFP-LC3 cells were treated with hypoxia (2 % O2) for 3 days.

Chemical inhibition of autophagy flux by chloroquine (CQ) suppresses hypoxia-induced NED in LNCaP cells(A) LNCaP cells were treated with hypoxia (2 % O2) in the absence or presence of 50 μM CQ for 3 days.

Knockdown of beclin 1 suppresses hypoxia-induced NED(A) LNCaP-TR-shBeclin1 cells were treated with 1 μg/mL Dox to induce beclin 1 knockdown for 4 days under normoxia and hypoxia (2 % O2).

Disruption of autophagy by treatment with CiQ derivativesA.

Disruption of autophagy by treatment with CiQ derivativesA.

Induction of apoptosis by treatment with CiQ derivativesA.

Induction of lysosome dysfunction by treatment with CiQ derivativesA and B.

Differential expression of CDK1 pTyr15 in colorectal cancer cells and tissues.

Senataxin is SUMOylated at the XY chromosomes in spermatocytes.

120 Abbildungen

105 Referenzen

Produktnummer ABIN2857072

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

TCF7L1 Antikörper (N-Term)

TCF7L1 Reaktivität: Human WB, ChIP-seq, ChIP, CUT&RUN Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1) (N-Term) antibody (ABIN6972849)

Western blot of TCF7L1 / TCF3 antibody.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

Alignment tracks from CUT&RUN targeting TCF7L1 in HEK293T cells using anti-TCF7L1 antibody ABIN6972849 (1).

6 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN6972849

663,08 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 2 bis 4 Werktagen

Independently Validated

HDAC1 Antikörper

KO Validated HDAC1 Reaktivität: Human WB, IF, ICC, IP, IHC (p), ChIP, IHC (fro), IHC (wm) Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

WB Image HDAC1 antibody detects HDAC1 protein by western blot analysis.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

IHC-P Image Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1, antibody(10 μg/ml).

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.

ICC/IF Image HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.

ChIP Image HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1, antibody at 1:200 dilution.

IP Image HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

SAHA affects the expression of suvivin and XIAP at the transcriptional level.

HDAC1/2 are robustly upregulated in SCs after lesion.

HDAC1/2 are robustly upregulated in SCs after lesion.

Overexpression of peptidase inhibitor 16 (PI16) attenuates angiotensin II (Ang II)-induced fibrotic gene expression and histone 3 modification in NRCFs.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

Alignment tracks from CUT&RUN targeting HDAC1 in Mouse fore limb (11.

22 Abbildungen

7 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2854776

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

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Lieferung in 7 bis 10 Werktagen

Independently Validated

CBP Antikörper (C-Term)

KO Validated CREBBP Reaktivität: Human WB, IF, IP, ICC, IHC (p), ChIP Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at 1:1000

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IP Image CBP antibody immunoprecipitates CBP protein in IP experiments.

ChIP Image CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP , antibody at 1:200 dilution.

11 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN2854987

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Independently Validated

Calretinin Antikörper

CALB2 Reaktivität: Human WB, IF, IHC (p), ICC, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN2855596

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 7 bis 10 Werktagen

Independently Validated

STAT3 Antikörper (C-Term)

KO Validated STAT3 Reaktivität: Human WB, IF, ICC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.

WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , 7.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3, antibody at 1:200 dilution.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1 (ERK-1), c-Jun N-terminal kinase 1 (JNK1), JNK2, and p38 in lung tissues of C57BL/6 mice.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of STAT3, ERK1, JNK1, JNK2, and p38 in liver tissues of C57BL/6 mice.

MLN8237 treatment resulted in abnormal activation of Akt/Stat3 pathway.

10 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN2855865

583,17 €

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100 μL

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Lieferung in 7 bis 10 Werktagen

Independently Validated

RAD21 Antikörper (Center)

RAD21 Reaktivität: Human, Maus WB, IF, IP, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-RAD21 Homolog (RAD21) (Center) antibody (ABIN2856242)

Cleavage Under Targets and Release Using Nuclease validation image for anti-RAD21 Homolog (RAD21) (Center) antibody (ABIN2856242)

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.

IHC-P Image Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using RAD21, antibody at 1:750 dilution.

IP Image Immunoprecipitation of RAD21 protein from Jurkat whole cell extracts using 5 μg of RAD21 antibody, Western blot analysis was performed using RAD21 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image RAD21 antibody detects RAD21 protein at nucleus by immunofluorescent analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RAD21, antibody at 1:750 dilution.

IHC-P Image RAD21 antibody detects RAD21 protein at nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis.

WB Image RAD21 antibody detects RAD21 protein by western blot analysis.

9 Abbildungen

2 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN2856242

583,17 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

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Lieferung in 7 bis 10 Werktagen

Independently Validated

MAP2K5 Antikörper (AA 251-350)

MAP2K5 Reaktivität: Human, Maus, Ratte WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Immunohistochemistry validation image for anti-Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 251-350) antibody (ABIN754183)

Mouse heart lysates probed with Rabbit Anti-MEK5 Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029644:Formalin-fixed and paraffin embedded human breast myoepithelial and glandular tissue labeled with Anti-MEK5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-MEK5/MAP2K5 Polyclonal Antibody, Unconjugated (ABIN754183) at 1:200 followed by conjugation to the secondary antibody and DAB staining

7 Abbildungen

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Independent Validation

1 Validierung

Produktnummer ABIN754183

357,70 €

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100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

Elastin Antikörper (AA 681-786)

ELN Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p), FACS Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Elastin (ELN) (AA 681-786) antibody (ABIN734003)

Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 overnight at 4 °C.

Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (ABIN734003) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated (ABIN734003) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (ABIN734003) at 1:1000 4 °C temperature overnight 4 °C followed by conjugation to secondary antibody.

Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody

8 Abbildungen

4 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN734003

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

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Lieferung in 8 bis 12 Werktagen

Independently Validated

RAF1 Antikörper (AA 31-130)

RAF1 Reaktivität: Human ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Immunohistochemistry validation image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody (ABIN733208)

Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-c-RafRaf1 Polyclonal Antibody, Unconjugated (ABIN733208) followed by conjugation to the secondary antibody and DAB staining

Images provided the Independent Validation Program (badge number 29651)Formalin-fixed and paraffin embedded human colon labeled with Rabbit Anti-c-Raf/Raf1 Polyclonal Antibody (ABIN733208) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

6 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN733208

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

GDNF Antikörper (AA 121-211)

GDNF Reaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen WB, FACS, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 121-211) antibody (ABIN736536)

Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyclonal Antibody, unconjugated (ABIN736536) at 1: 200 followed by incubation with conjugated secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal Antibody, Unconjugated (ABIN736536) at 1:200 in 4 °C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

6 Abbildungen

1 reference

Independent Validation

1 Validierung

Produktnummer ABIN736536

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

Tissue factor Antikörper (AA 32-100)

F3 Reaktivität: Human, Maus, Ratte, Schwein WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Immunohistochemistry validation image for anti-Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 32-100) antibody (ABIN708086)

Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029589:Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD142 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat lung labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (ABIN708086) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

10 Abbildungen

7 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN708086

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

AQP2 Antikörper (AA 171-271)

AQP2 Reaktivität: Human, Ratte, Maus WB, ELISA, IF (p), IF (cc), IHC (p), ICC, FACS, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 171-271) antibody (ABIN707576)

Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C.

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (ABIN707576) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody FITC Conjugated Secondary Antibody.

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated was used to stain the cell nuclei.

12 Abbildungen

2 Referenzen

Independent Validation

1 Validierung

Produktnummer ABIN707576

357,70 €

Zzgl. Versandkosten 20,00 € und MwSt

100 μL

Lieferung nach: Deutschland

Lieferung in 8 bis 12 Werktagen

Independently Validated

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