Complement Factor H Antikörper (AA 20-270)

Produktnummer ABIN3023097

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Complement Factor H Antikörper wird verwendet zum Nachweis von Complement Factor H in Proben von Human. Er wurde validiert für WB, IHC und IF. Der Antikörper wurde zudem unabhängig validiert.

Kurzübersicht für Complement Factor H Antikörper (AA 20-270) (ABIN3023097)

Target: Complement Factor H (CFH)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Complement Factor H Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Complement Factor H Antikörper

Bindungsspezifität: AA 20-270

Sequenz: DCNELPPRRN TEILTGSWSD QTYPEGTQAI YKCRPGYRSL GNVIMVCRKG EWVALNPLRK CQKRPCGHPG DTPFGTFTLT GGNVFEYGVK AVYTCNEGYQ LLGEINYREC DTDGWTNDIP ICEVVKCLPV TAPENGKIVS SAMEPDREYH FGQAVRFVCN SGYKIEGDEE MHCSDDGFWS KEKPKCVEIS CKSPDVINGS PISQKIIYKE NERFQYKCNM GYEYSERGDA VCTESGWRPL PSCEEKSCDN P

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Polyclonal Antibodies

Aufreinigung: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Complement factor H (NP_001014975.1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #101403 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101403

Datum: 19.07.2017

Antigen: Commplement Factor H

Chargennummer: 4390101

Validierte Anwendung: Immunofluorescence

Positivkontrolle: MRL/lpr lupus mice (14 weeks of age)

Negativkontrolle: FH knockout MRL/lpr lupus mice

Bewertung: Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.

Validierungsbilder

IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Protokoll

Primärantikörper: ABIN3023097

Sekundärantikörper: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Referenzen für anti-Complement Factor H Antikörper (ABIN3023097)

Cheng, Giguere, Silverstein, Conza, Seddon, Kim, Iwata, Mueller, Punzo: "Role of alpha-1 antitrypsin in Bruch's membrane integrity." in: Scientific reports, Vol. 15, Issue 1, pp. 12223, (2025) (PubMed).

Cheng, Malachi, Cipi, Ma, Brush, Agbaga, Punzo: "HK2 Mediated Glycolytic Metabolism in Mouse Photoreceptors Is Not Required to Cause Late Stage Age-Related Macular Degeneration-Like Pathologies." in: Biomolecules, Vol. 11, Issue 6, (2021) (PubMed).

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: CFH

Hintergrund: This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,CFH,AHUS1,AMBP1,ARMD4,ARMS1,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Immunology & Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,CFH

Molekulargewicht: 51 kDa/139 kDa

Gen-ID: 3075

UniProt: P08603

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin