IKBKG ELISA Kit
IKBKG
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690893
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Target
Alle IKBKG ELISA Kits anzeigen
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IKBKG
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))
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Reaktivität
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Human
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Human IKK-gamma/NEMO/FIP-3 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Human IKK-gamma.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product IKBKG ELISA Kit
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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Target
Alle IKBKG ELISA Kits anzeigen
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IKBKG
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))
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Andere Bezeichnung
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IKK-gamma (NEMO/FIP-3) (IKBKG Produkte)
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Synonyme
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AMCBX1 ELISA Kit, FIP-3 ELISA Kit, FIP3 ELISA Kit, Fip3p ELISA Kit, IKK-gamma ELISA Kit, IP ELISA Kit, IP1 ELISA Kit, IP2 ELISA Kit, IPD2 ELISA Kit, NEMO ELISA Kit, ZC2HC9 ELISA Kit, 1110037D23Rik ELISA Kit, AI848108 ELISA Kit, AI851264 ELISA Kit, AW124339 ELISA Kit, IKK[g] ELISA Kit, Nemo ELISA Kit, nemo ELISA Kit, fj33c07 ELISA Kit, wu:fj33c07 ELISA Kit, zgc:113492 ELISA Kit, MGC97885 ELISA Kit, IKBKG ELISA Kit, IKBKG_1 ELISA Kit, IKBKG_2 ELISA Kit, CG16910 ELISA Kit, DmIKK-gamma ELISA Kit, DmIKKgamma ELISA Kit, Dmel\\CG16910 ELISA Kit, Dmikkgamma ELISA Kit, IKK ELISA Kit, IKK[[gamma]] ELISA Kit, IKKg ELISA Kit, IKKgamma ELISA Kit, KEY ELISA Kit, Kenny ELISA Kit, Key ELISA Kit, dIKK ELISA Kit, dIKK-gamma ELISA Kit, dmIKKgamma ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit gamma ELISA Kit, inhibitor of kappaB kinase gamma ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog ELISA Kit, kenny ELISA Kit, IKBKG ELISA Kit, Ikbkg ELISA Kit, ikbkg ELISA Kit, ikbkg.L ELISA Kit, IKBKG_1 ELISA Kit, key ELISA Kit
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Gen-ID
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8517
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UniProt
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Q9Y6K9
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Pathways
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NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteine
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