IL6RA Protein (AA 20-365) (His-Avi Tag)
-
- Target Alle IL6RA Proteine anzeigen
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 20-365
-
Spezies
- Human
-
Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses IL6RA Protein ist gelabelt mit His-Avi Tag.
- Verwendungszweck
- Human IL-6 R alpha/CD126 Protein
- Sequenz
- Leu20-Pro365
- Produktmerkmale
- Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-6, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.25μg/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
-
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
-
- Target
- IL6RA (Interleukin 6 Receptor, alpha (IL6RA))
- Andere Bezeichnung
- IL-6R alpha (IL6RA Produkte)
- Synonyme
- IL6R1 Protein, Il6ra Protein, CD126 Protein, IL-6R-1 Protein, IL-6RA Protein, IL6Q Protein, IL6RA Protein, IL6RQ Protein, gp80 Protein, IL-6R Protein, Il6r Protein, interleukin 6 receptor Protein, interleukin 6 receptor, alpha Protein, Il6r Protein, IL6R Protein, Il6ra Protein
- Hintergrund
- The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.
- Molekulargewicht
- 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signalweg, Autophagie, Growth Factor Binding, Cancer Immune Checkpoints
-