MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448)

Details for Product anti-FRAP1 Antibody No. ABIN362262, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin kinase
  • mechanistic target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
pSer2448
67
31
29
22
21
18
16
15
15
11
7
7
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
408
221
212
16
8
6
6
6
6
5
4
3
2
1
1
1
Wirt
Kaninchen
356
49
3
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
10
10
9
5
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunohistochemistry (IHC)
295
155
128
69
59
52
34
20
16
8
8
7
3
3
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Peptide sequence around phosphorylation site of pSer2448 (T-D-S (p) -Y-S) derived from Human mTOR. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotyp IgG
Spezifität The antibody detects endogenous level of mTOR only when phosphorylated at serine 2448.
Reinigung The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmide, Primers & weitere MTOR products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung mTOR (FRAP1 Antibody Abstract)
Hintergrund Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Molekulargewicht 289 kDa
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Applikations-hinweise Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Produkt verwendet in: Macias, Blazejczyk, Kazmierska, Caban, Skalecka, Tarkowski, Rodo, Konopacki, Jaworski: "Spatiotemporal characterization of mTOR kinase activity following kainic acid induced status epilepticus and analysis of rat brain response to chronic rapamycin treatment." in: PLoS ONE, Vol. 8, Issue 5, pp. e64455, 2013 (PubMed).

Ning, Huang, Dong, Sun, Zhang, Xu, Li: "5-Aza-2'-deoxycytidine inhibited PDGF-induced rat airway smooth muscle cell phenotypic switching." in: Archives of toxicology, Vol. 87, Issue 5, pp. 871-81, 2013 (PubMed).

Allgemeine Veröffentlichungen Albanell, Dalmases, Rovira, Rojo: "mTOR signalling in human cancer." in: Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, Vol. 9, Issue 8, pp. 484-93, 2007 (PubMed).

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