p53 Antikörper

Produktnummer ABIN1842579

Produktdetails anti-p53 Antikörper

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p53 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: P53 Antibody (5H7B9), mAb, Mouse detects endogenous levels of p53 protein. Special reactivity with p53 isoforms of p53 antibody clones are tested by Western blot and described in the below table.

Produktmerkmale: p53 Antibody (5H7B9), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant wild type p53 (wt p53).

Aufreinigung: Protein A affinity column

Immunogen: Human recombinant wild type p53

Klon: 5H7B9

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0. 05-0. 1 µg/mL
Western blot: 1 µg/mL
Flow cytometry: 1-3 µg for 1 x 10^6 cells
Other applications:User-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Konzentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.

Referenzen für anti-p53 Antikörper (ABIN1842579)

Heyduk, Heyduk: "Ribosome display enhanced by next generation sequencing: a tool to identify antibody-specific peptide ligands." in: Analytical biochemistry, Vol. 464, pp. 73-82, (2014) (PubMed).

Details zu p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Andere Bezeichnung: p53 (TP53 Produkte)

Synonyme: BCC7 antikoerper, LFS1 antikoerper, P53 antikoerper, TRP53 antikoerper, p53 antikoerper, Trp53 antikoerper, brp53 antikoerper, drp53 antikoerper, etID22686.5 antikoerper, fb40d06 antikoerper, wu:fb40d06 antikoerper, zgc:111919 antikoerper, Tp53 antikoerper, bbl antikoerper, bfy antikoerper, bhy antikoerper, p44 antikoerper, tp53 antikoerper, Xp53 antikoerper, TP53 antikoerper, tumor protein p53 antikoerper, cellular tumor antigen p53 antikoerper, transformation related protein 53 antikoerper, tumor protein p53 L homeolog antikoerper, TP53 antikoerper, CpipJ_CPIJ002758 antikoerper, Tp53 antikoerper, tp53 antikoerper, Trp53 antikoerper, tp53.L antikoerper

Hintergrund: The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. It is situated at the crossroads of signalling pathways that are essential for cell growth regulation and apoptosis. In normal unstressed cells, the level of p53 protein is downregulated via the binding of proteins such as MDM2, COP1, PIRH2 or JNK that promote p53 degradation via the ubiquitin/proteasome pathway. As most of these genes are up regulated by p53, this lead to a regulation loop that will keep p53 level very low in a normal cells. Alternative splicing of the human p53 gene gives rise of p53 beta, p53 gamma, Delta 40p53 (also known as p44, p47 or delat N p53), Delta 40p53 beta, Delta 40 p53gamma, Delta 133p53, Delta 133p53 beta, and Delta 133p53 gamma

Pathways: p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie, Warburg Effekt