AKT1 Antikörper (AA 466-480)

Produktnummer ABIN126858

Produktdetails anti-AKT1 Antikörper

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Bindungsspezifität: AA 466-480

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AKT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: This antibody specifically recognizes the C-terminus of protein kinase B (aa 466-480).

Produktmerkmale: Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

Aufreinigung: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Klon: 5C10

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 0.05 μg/mL. Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: HepG2 untreated (See Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT)

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Andere Bezeichnung: AKT1 / PKB (AKT1 Produkte)

Synonyme: AKT antikoerper, CWS6 antikoerper, PKB antikoerper, PKB-ALPHA antikoerper, PRKBA antikoerper, RAC antikoerper, RAC-ALPHA antikoerper, Akt antikoerper, Rac antikoerper, PKB/Akt antikoerper, PKBalpha antikoerper, AKT/PKB antikoerper, AKT1 antikoerper, Akt/PKB antikoerper, Akt1 antikoerper, CG4006 antikoerper, D-Akt antikoerper, DAKT1 antikoerper, DAKT1/PKB antikoerper, DAkt antikoerper, DAkt1 antikoerper, DPKB antikoerper, DRAC-PK antikoerper, DRAC-PK66 antikoerper, DRAC-PK85 antikoerper, Dakt antikoerper, Dakt1 antikoerper, Dmel\\CG4006 antikoerper, Dpkb antikoerper, PKB/AKT antikoerper, PKB/dAKT antikoerper, RacPK antikoerper, akt antikoerper, akt1 antikoerper, dAKT antikoerper, dAKT/dPKB antikoerper, dAKT1 antikoerper, dAkt antikoerper, dAkt/PKB antikoerper, dAkt1 antikoerper, dPKB antikoerper, dakt antikoerper, dakt1 antikoerper, l(3)04226 antikoerper, l(3)89Bq antikoerper, p-Akt antikoerper, pAkt antikoerper, ACT-5 antikoerper, akt-1 antikoerper, pkb antikoerper, v-akt antikoerper, v-akt1 antikoerper, xAct antikoerper, ATAKT1 antikoerper, F18A8.2 antikoerper, F18A8_2 antikoerper, K+ transporter 1 antikoerper, POTASSIUM TRANSPORTER antikoerper, AKT serine/threonine kinase 1 antikoerper, thymoma viral proto-oncogene 1 antikoerper, CG4006 gene product from transcript CG4006-RE antikoerper, actin beta antikoerper, v-akt murine thymoma viral oncogene homolog 1 S homeolog antikoerper, v-akt murine thymoma viral oncogene homolog 1 antikoerper, K+ transporter 1 antikoerper, Serine/threonine-protein kinase akt-1 antikoerper, AKT1 antikoerper, Akt1 antikoerper, ACTB antikoerper, akt1.S antikoerper, akt1 antikoerper, KT1 antikoerper, akt-1 antikoerper

Hintergrund: The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase

Molekulargewicht: 60 kDa

Gen-ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, AMPK Signaling, Interferon-gamma Pathway, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effekt