LAG3 Protein (AA 23-434) (His-Avi Tag,Biotin)

Produktnummer ABIN7275115

Produktdetails

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-434

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LAG3 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human LAG3/CD223 Protein

Sequenz: Leu23-Gly434

Produktmerkmale: Recombinant Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu23-Gly434.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-LAG3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: LAG3 (LAG3 Produkte)

Synonyme: CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein

Hintergrund: LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.

Molekulargewicht: 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints