LAG3 Protein (His tag)

Produktnummer ABIN7320943

Produktdetails

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Cynomolgus

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses LAG3 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Cynomolgus LAG-3/CD223 Protein (His Tag)

Sequenz: Pro23-Leu450(Pro74)

Produktmerkmale: Recombinant Cynomolgus LAG-3 is produced by our Mammalian expression system and the target gene encoding Pro23-Leu450(Pro74) is expressed with a 8His tag at the N-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus LAG-3-His at 2μg/ml(100 μl/well) can bind Human FGL1-Fc. The ED50 of Cynomolgus LAG-3-His is 5.8 ug/ml .

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: LAG-3/CD223 (LAG3 Produkte)

Synonyme: CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein

Hintergrund:

Background: Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3',s main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.

Synonym: Lymphocyte Activating 3, LAG3, LAG-3, CD223

Molekulargewicht: 47.3 kDa

NCBI Accession: XP_005570011

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints