LAG3 Protein (AA 23-450) (hFc Tag)

Produktnummer ABIN7275114

Dieses Recombinant LAG3-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für LAG3 Protein (AA 23-450) (hFc Tag) (ABIN7275114)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 23-450

Aufreinigungstag / Konjugat: Dieses LAG3 Protein ist gelabelt mit hFc Tag.

Verwendungszweck: Human LAG3/CD223 Protein

Sequenz: Leu23-Leu450

Produktmerkmale: Recombinant Human LAG3/CD223 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu23-Leu450.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LAG3, hFc Tag with the EC50 of 75.1ng/ml determined by ELISA.,Human LAG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%,The purity of Human LAG3 is greater than 90% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: LAG3

Hintergrund: LAG-3, is a protein which in humans is encoded by the LAG3 gene, which is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders.

Molekulargewicht: 72.4 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Bis-Tris PAGE result.

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints