IL-1 beta Protein (AA 117-269) (His tag)

Produktnummer ABIN6239741

Produktdetails

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Biologische Aktivität: Active

Proteineigenschaft: AA 117-269

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-1 beta Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: Interleukin-1 beta belongs to the interleukin 1 cytokine family, which plays a critical role in inflammation, immunity, antiviral responses, and a variety of diseases. It has been reported that IL-1β-induced IL-6 production is mediated by both PI3K and IRAK4 in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (1ng/mL, 10ng/mL) of IL1-β for 4h and IL-6 levels in the cell supernatant were determined by ELISA. IL-6 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL1-β, the data was shown in Table 1 and Figure 1. Sample O.D. value Corrected Concentration of IL-6(cell supernatant of A549 cells) (ng/mL) stimulated with IL-1β(1ng/mL) 1.04 0.975 61.71 stimulated with IL-1β(10ng/mL) 0.966 0.901 56.77 unstimulated 0.309 0.244 12.44 Table 1. IL-6 levels in the cell supernatant of A549 cells up-regulated by IL1-β 80 60 40 20 0 0 1 10 rh IL-1β (ng/ml) Figure 1. IL-6 levels in the cell supernatant of A549 cells up-regulated by IL1-β.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.9

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: Interleukin 1 Beta (IL1b) (IL1B Produkte)

Synonyme: IL-1 Protein, IL1-BETA Protein, IL1F2 Protein, IL-1BETA Protein, IL1beta Protein, il1-b Protein, zgc:111873 Protein, IL-1B Protein, IL-1beta Protein, Il-1b Protein, IL1B Protein, IL-1 beta Protein, IL-1b Protein, interleukin 1 beta Protein, interleukin 1, beta Protein, IL1B Protein, il1b Protein, Il1b Protein

Hintergrund: Alternative Names: IL1-B, IL1-Beta, IL1F2, IL-1β, Interleukin-1 Family Member 2, Catabolin

Molekulargewicht: 22kDa

UniProt: P01584

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome