IL-6 Protein

Produktnummer ABIN2723740

Produktdetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-6 / IL6 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells is less than or equal to 0.1 ng/ml, corresponding to a specific activity of > 1 x 10^7units/mg.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: Interleukin-6,il6 (IL6 Produkte)

Synonyme: BSF2 Protein, HGF Protein, HSF Protein, IFNB2 Protein, IL-6 Protein, Il-6 Protein, ILg6 Protein, Ifnb2 Protein, il6 Protein, CHIL-6 Protein, interleukin 6 Protein, interleukin-6 Protein, IL6 Protein, Il6 Protein, il-6 Protein, IL-6 Protein

Hintergrund: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 20.9 kDa

NCBI Accession: NP_000591

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome