IL-1 beta Antikörper (N-Term)

Produktnummer ABIN799612

Produktdetails anti-IL-1 beta Antikörper

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Bindungsspezifität: N-Term

Reaktivität: Human, Hund, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-1 beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).

Aufreinigung: Purified IgG

Immunogen: Recombinant human IL1B / IL1 Beta.

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)

Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL1B (IL1B Produkte)

Synonyme: IL-1 antikoerper, IL1-BETA antikoerper, IL1F2 antikoerper, IL-1BETA antikoerper, IL1beta antikoerper, il1-b antikoerper, zgc:111873 antikoerper, IL-1B antikoerper, IL-1beta antikoerper, Il-1b antikoerper, IL1B antikoerper, IL-1 beta antikoerper, IL-1b antikoerper, interleukin 1 beta antikoerper, interleukin 1, beta antikoerper, IL1B antikoerper, il1b antikoerper, Il1b antikoerper

Hintergrund: Name/Gene ID: IL1B
Family: IL-1

Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta

Gen-ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome