Insulin Antikörper
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- Target Alle Insulin (INS) Antikörper anzeigen
- Insulin (INS)
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Reaktivität
- Schwein
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Wirt
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Meerschweinchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Insulin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID)
- Spezifität
- The reactivity of the antiserum is directed to Insulin as tested by ELISA and Immunoblotting. No reaction was obtained with other Swine serum proteins.
- Aufreinigung
- Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt Precipitation and purification of the IgG fraction by DEAE Chromatography
- Immunogen
- Insulin hormone is isolated from Porcine pancreas. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotyp
- IgG
- Top Product
- Discover our top product INS Primärantikörper
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- Applikationshinweise
- The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates in radioimmunoassay for the production of immunoconjugates with a selected marker to prepare immunoaffinity adsorbents by coupling to an artificial carrier in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent in Western blotting. This product is not pre-diluted. Recommended Working Dilutions : Histochemical and Cytochemical Use: are usually between 1/100-1/250. In ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- PBS, pH 7.2 Preservatives: None, as it may interfere with the antibody activity. No foreign proteins added
- Konservierungsmittel
- Azide free
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
- Haltbarkeit
- 12 months
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- Target
- Insulin (INS)
- Andere Bezeichnung
- insulin (INS Produkte)
- Synonyme
- IDDM2 antikoerper, ILPR antikoerper, IRDN antikoerper, MODY10 antikoerper, ins1 antikoerper, xins antikoerper, ins1-a antikoerper, Insulin antikoerper, AA986540 antikoerper, Ins-2 antikoerper, InsII antikoerper, Mody antikoerper, Mody4 antikoerper, proinsulin antikoerper, zgc:109842 antikoerper, igf2-A antikoerper, ins antikoerper, ins-a antikoerper, ins-b antikoerper, insulin antikoerper, insulin precursor antikoerper, insulin II antikoerper, preproinsulin antikoerper, insulin L homeolog antikoerper, insulin S homeolog antikoerper, INS antikoerper, INS-IGF2 antikoerper, ins antikoerper, Ins antikoerper, PIN antikoerper, Ins2 antikoerper, ins.L antikoerper, ins.S antikoerper
- Hintergrund
- Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas. It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues. Insulin has a molecular weight of 6,000 and contains two peptide chains linked by two disulphide bonds.
- UniProt
- P01315
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagie, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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