eIF4EBP1 Antikörper (AA 101-118)
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- Target Alle eIF4EBP1 (EIF4EBP1) Antikörper anzeigen
- eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
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Bindungsspezifität
- AA 101-118
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Reaktivität
- Human, Maus, Ratte, Kaninchen, Hamster
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser eIF4EBP1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Gel Shift (GS)
- Marke
- IHC-plus™
- Spezifität
- Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Top Product
- Discover our top product EIF4EBP1 Primärantikörper
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- Applikationshinweise
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Approved: GS, IHC, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
- Handhabung
- avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
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- Target
- eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
- Andere Bezeichnung
- EIF4EBP1 / 4EBP1 (EIF4EBP1 Produkte)
- Synonyme
- 4E-BP1 antikoerper, 4EBP1 antikoerper, BP-1 antikoerper, PHAS-I antikoerper, 4e-bp1 antikoerper, AA959816 antikoerper, 153432_at antikoerper, 43-BP antikoerper, 4E-BP antikoerper, 4EBP antikoerper, 4e-bp antikoerper, 4eBP antikoerper, 4ebp antikoerper, BcDNA.HL08053 antikoerper, BcDNA:HL08053 antikoerper, CG8846 antikoerper, CT8705 antikoerper, Dm 4E-BP antikoerper, Dmel\\CG8846 antikoerper, Phas1 antikoerper, Thor/EIF4EBP antikoerper, anon-WO0172774.158 antikoerper, d4E-BP antikoerper, d4E-BP1 antikoerper, d4EBP antikoerper, d4e-bp antikoerper, d4eBP antikoerper, dthor antikoerper, eIF4E-BP antikoerper, l(2)06270 antikoerper, l(2)k07736 antikoerper, l(2)k13517 antikoerper, pp20 antikoerper, thor antikoerper, EIF4EBP1 antikoerper, fc04h09 antikoerper, wu:fc04h09 antikoerper, zgc:64137 antikoerper, eukaryotic translation initiation factor 4E binding protein 1 antikoerper, CG8846 gene product from transcript CG8846-RA antikoerper, EIF4EBP1 antikoerper, Eif4ebp1 antikoerper, Thor antikoerper, eif4ebp1 antikoerper
- Hintergrund
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Name/Gene ID: EIF4EBP1
Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1 - Gen-ID
- 1978
- UniProt
- Q13541
- Pathways
- MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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