GLI2 Antikörper (Internal Region)

Produktnummer ABIN401282

Produktdetails anti-GLI2 Antikörper

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Bindungsspezifität: Internal Region

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLI2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against Gli-2 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rat (100 %).
Species reactivity (tested):Mouse.

Aufreinigung: Immunoafiinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/15,000-1/60,000. Western blot: 1/500-1/2,000. Immunohistochemistry on Paraffin Sections: 2-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.02 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Andere Bezeichnung: GLI2 (GLI2 Produkte)

Synonyme: HPE9 antikoerper, THP1 antikoerper, THP2 antikoerper, GLI2/GLI4 antikoerper, GLI3 antikoerper, AW546128 antikoerper, GLI family zinc finger 2 antikoerper, GLI-Kruppel family member GLI2 antikoerper, GLI2 antikoerper, Gli2 antikoerper

Hintergrund: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, post-axial polydactyly types A1 and B. Expression has been reported for this mRNA in human testis, myometrium, kidney, lung, glioblastomas, and embryonal cell carcinomas. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2

Gen-ID: 2736

NCBI Accession: NP_005261

UniProt: P10070

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis