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GLI2 Antikörper (GLI Family Zinc Finger 2) Internal Region Primary Antibody

GLI2 Reaktivität: Schimpanse, Human IHC, ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Pubmed (5 references)
Produktnummer ABIN129627
$658.50
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
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Lieferung in 1 bis 2 Werktagen
  • Target Alle GLI2 Antikörper anzeigen
    GLI2
    Bindungsspezifität
    • 9
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 47
    • 15
    • 6
    • 2
    • 1
    • 1
    Schimpanse, Human
    Wirt
    • 41
    • 7
    • 2
    Kaninchen
    Klonalität
    • 48
    • 2
    Polyklonal
    Konjugat
    • 20
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser GLI2 Antikörper ist unkonjugiert
    Applikation
    • 24
    • 21
    • 13
    • 6
    • 6
    • 5
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Spezifität
    This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
    Produktmerkmale
    Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.
    Sterilität
    Sterile filtered
    Immunogen
    This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human Gli-2 (isoform a).
    Immunogen Type: Peptide
    Isotyp
    IgG
  • Applikationshinweise
    This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
    Kommentare

    Gene Name: GLI2

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Haltbarkeit
    12 months
  • Gonnissen, Isebaert, Perneel, McKee, Van Utterbeeck, Lerut, Verrill, Bryant, Joniau, Muschel, Haustermans: "Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients." in: The American journal of pathology, Vol. 188, Issue 3, pp. 795-804, (2019) (PubMed).

    Gonnissen, Isebaert, McKee, Dok, Haustermans, Muschel: "The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 51, pp. 84286-84298, (2018) (PubMed).

    Gonnissen, Isebaert, Perneel, McKee, Verrill, Bryant, Van Utterbeeck, Lerut, Haustermans, Muschel: "Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer." in: BMC cancer, Vol. 17, Issue 1, pp. 634, (2018) (PubMed).

    Gonnissen, Isebaert, McKee, Muschel, Haustermans: "The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).

    Roessler, Ermilov, Grange, Wang, Grachtchouk, Dlugosz, Muenke: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, (2005) (PubMed).

  • Target
    GLI2
    Andere Bezeichnung
    Gli-2 (GLI2 Produkte)
    Synonyme
    HPE9, THP1, THP2, GLI2/GLI4, GLI3, AW546128, GLI family zinc finger 2, GLI-Kruppel family member GLI2, GLI2, Gli2
    Hintergrund
    Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.
    Synonyms: Gli-2, Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein
    Gen-ID
    2736
    NCBI Accession
    NP_005261
    UniProt
    P10070
    Pathways
    Hedgehog Signalweg, Dopaminergic Neurogenesis
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