Protein A column, followed by peptide affinity purification
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T69 of human EIF4EBP1.
ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Target
eIF4EBP1 (EIF4EBP1)
(Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
EIF4EBP1 is one member of a family of translation repressor proteins. It directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1