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GLI2 Antikörper (GLI Family Zinc Finger 2) Internal Region Primary Antibody

Verified GLI2 Reaktivität: Human ELISA, IHC Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185585
$410.72
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 7 Werktagen
  • Target Alle GLI2 Antikörper anzeigen
    GLI2
    Bindungsspezifität
    • 9
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 46
    • 15
    • 6
    • 2
    • 2
    • 1
    Human
    Wirt
    • 42
    • 6
    • 2
    Ziege
    Klonalität
    • 48
    • 2
    Polyklonal
    Konjugat
    • 20
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser GLI2 Antikörper ist unkonjugiert
    Applikation
    • 25
    • 20
    • 13
    • 6
    • 6
    • 5
    • 1
    ELISA, Immunohistochemistry (IHC)
    Verwendungszweck
    GLI2
    Sequenz
    DLKEDLDRDD CKQE
    Spezifität
    This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
    Homologie
    Human, Mouse
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.
    Isotyp
    IgG
  • Applikationshinweise

    DS WB Results: Preliminary experiments gave an approx 55 kDa band in Human Bone Marrow and Ovary lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.

    DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:2000.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    GLI2
    Andere Bezeichnung
    GLI2 (GLI2 Produkte)
    Synonyme
    HPE9, THP1, THP2, GLI2/GLI4, GLI3, AW546128, GLI family zinc finger 2, GLI-Kruppel family member GLI2, GLI2, Gli2
    Hintergrund
    GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2
    Gen-ID
    2736
    NCBI Accession
    NP_005261
    Pathways
    Hedgehog Signalweg, Dopaminergic Neurogenesis
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