NFKB1 Antikörper (N-Term)

Produktnummer ABIN116520

Produktdetails anti-NFKB1 Antikörper

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFKB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Spezifität: Anti-NFkB p50 may react non-specifically with other proteins. Control peptide (#R1008PEP) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1). In immunoblot no reaction was observed against the analogous Mmouse protein.

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH)

Anwendungsinformationen

Applikationshinweise: Suitable for use in Immunoprecipitation, Immunoblotting (1/500-1/2,000), ELISA(1/5,000-1/25,000) and Supershift assays. This product was assayed by Immunoblot and found to be reactive against Human NFkBp50 (NFKB1) at a dilution of 1/1000 followed by reaction with Peroxidase conjugatedanti-Rabbit IgG [H&L] R1364HRP. Anti-Human NFkB p50 (NFKB1) is suitable for the detectionby Immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105. This product was also tested in a Gel Supershift assay and found to be reactive againstp50: p50 homodimers and p: 50: p65 heterodimers using 0.5 to 1.0 μL per assay. Gel (Super) Shift Information: In general, NFkB gel shift assays are assembled in 20 μL reactions containing 0.28 pmolesNFkB oligo in 10 mM Tris ( pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10 % glycerol. Some procedures specify the addition of 0.05 % NP-40. When using purified protein,250-300 ng should be sufficient to produce a gel shifted complex, while 10 ?g HeLa nuclear

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 80.0 mg/mL (by Refractometry)

Vorsichtsmaßnahmen: This product contains sodium azide : a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NFKB1

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Andere Bezeichnung: NF-kB p105 / p50 (NFKB1 Produkte)

Synonyme: GKLP antikoerper, NKTL antikoerper, NTKL antikoerper, P105 antikoerper, TAPK antikoerper, TEIF antikoerper, TRAP antikoerper, EBP-1 antikoerper, KBF1 antikoerper, NF-kB1 antikoerper, NF-kappa-B antikoerper, NF-kappaB antikoerper, NFKB-p105 antikoerper, NFKB-p50 antikoerper, NFkappaB antikoerper, p105 antikoerper, p50 antikoerper, NF-kB antikoerper, NF-KB1 antikoerper, ebp-1 antikoerper, kbf1 antikoerper, nf-kappa-b antikoerper, nf-kappaB antikoerper, nfkb-p105 antikoerper, nfkb-p50 antikoerper, NF-kappaB1 antikoerper, p50/p105 antikoerper, BICDR-1 antikoerper, bicdr1 antikoerper, ccdc64 antikoerper, SCY1 like pseudokinase 1 antikoerper, nuclear factor kappa B subunit 1 antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 L homeolog antikoerper, nuclear factor NF-kappa-B p105 subunit antikoerper, nucleoporin 107 antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antikoerper, SCYL1 antikoerper, NFKB1 antikoerper, Nfkb1 antikoerper, nfkb1.L antikoerper, CpipJ_CPIJ012236 antikoerper, Nup107 antikoerper, nfkb1 antikoerper

Hintergrund: NFκB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52(NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: EBP-1, EBP1, KBF1, NF-kappa-B p50, NFKB1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Gen-ID: 4790

NCBI Accession: NP_001158884

UniProt: P19838

Pathways: p53 Signalweg, NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteine