IFNA Protein (AA 25-437) (His tag)

Produktnummer ABIN7454143

Produktdetails

Target: IFNA (Interferon alpha (IFNA))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-437

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IFNA Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus IFN alpha/beta R1 Protein

Sequenz: Ala25-Lys437

Produktmerkmale: Recombinant Cynomolgus IFN alpha/beta R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Lys437.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus IFNAR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IFNAR1 Antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IFNA (Interferon alpha (IFNA))

Andere Bezeichnung: IFN alpha (IFNA Produkte)

Synonyme: IFN-alphaO Protein, IFN-ALPHA-1 Protein, IFN1@ Protein, Ifa Protein, Ifa8 Protein, interferon alpha 16 Protein, interferon, alpha 1 Protein, interferon alpha Protein, interferon-alpha Protein, IFNA16 Protein, IFNA1 Protein, Ifna Protein, IFNA Protein

Hintergrund: IFN-alpha / beta R1, also known as IFNAR1, belongs to the class II cytokine receptor family of proteins. Class II cytokine receptors form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions.Functions in general as heterodimer with IFNAR2. Type I interferon binding activates the JAK-STAT signaling cascade, and triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and the IFNR alpha- and beta-subunits themselves.

Molekulargewicht: 48.54 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

UniProt: A0A7N9D7J0

Pathways: JAK-STAT Signalweg, TLR Signalweg, Hepatitis C, Inflammasome