CSF1R Protein (His-Avi Tag,Biotin)

Produktnummer ABIN7274428

Produktdetails

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CSF1R Protein ist gelabelt mit His-Avi Tag,Biotin.

Sequenz: Ile20-Glu512

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CSF R Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 45.4ng/ml determined by ELISA. The affinity constant of 0.81 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Andere Bezeichnung: M-CSF R (CSF1R Produkte)

Synonyme: AI323359 Protein, CD115 Protein, CSF-1R Protein, Csfmr Protein, Fim-2 Protein, Fms Protein, M-CSF-R Protein, M-CSFR Protein, C-FMS Protein, CSFR Protein, FIM2 Protein, FMS Protein, HDLS Protein, PDGFRB Protein, MGC89146 Protein, CSF-1-R Protein, c-fms Protein, mrfms Protein, CSF1 Protein, M-CSF Protein, csf1r Protein, fms Protein, panther Protein, colony stimulating factor 1 receptor Protein, macrophage colony-stimulating factor receptor Protein, colony stimulating factor 1 receptor, a Protein, Csf1r Protein, CSF1R Protein, m-csfr Protein, csf1r Protein, csf1ra Protein

Hintergrund: CD115, CSF1R, CSF-1-R, CSFR, FMSFIM2, M-CSF R, MCSFR, M-CSFR, C-FMS, FIM2, FMS, HDLS,CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molekulargewicht: 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signalweg, Inositol Metabolic Process, Cell-Cell Junction Organization