CSF1R Protein (AA 99-596) (His tag)

Produktnummer ABIN7274422

Produktdetails

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein-Typ: Recombinant

Proteineigenschaft: AA 99-596

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CSF1R Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus M-CSF R/CSF1R/CD115 Protein

Sequenz: Ile99-Pro596

Produktmerkmale: Recombinant Cynomolgus M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile99-Pro596.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus M-CSF R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. The affinity constant of 0.889 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Andere Bezeichnung: M-CSF R (CSF1R Produkte)

Synonyme: AI323359 Protein, CD115 Protein, CSF-1R Protein, Csfmr Protein, Fim-2 Protein, Fms Protein, M-CSF-R Protein, M-CSFR Protein, C-FMS Protein, CSFR Protein, FIM2 Protein, FMS Protein, HDLS Protein, PDGFRB Protein, MGC89146 Protein, CSF-1-R Protein, c-fms Protein, mrfms Protein, CSF1 Protein, M-CSF Protein, csf1r Protein, fms Protein, panther Protein, colony stimulating factor 1 receptor Protein, macrophage colony-stimulating factor receptor Protein, colony stimulating factor 1 receptor, a Protein, Csf1r Protein, CSF1R Protein, m-csfr Protein, csf1r Protein, csf1ra Protein

Hintergrund: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molekulargewicht: 56.3 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

UniProt: G7P8P1

Pathways: RTK Signalweg, Inositol Metabolic Process, Cell-Cell Junction Organization