TNFRSF1A Protein (His tag)

Produktnummer ABIN7320255

Produktdetails

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFRSF1A Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse TNFR1/TNFRSF1A Protein (His Tag)(Active)

Sequenz: Met 1-Ala 212

Produktmerkmale: A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was expressed, with a polyhistidine tag at the C-terminus.

Reinheit: > 96 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind  biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL.3. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind  biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL.4. Measured by its ability to inhibit the TNFα-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.5-1.5 μg/mL in the presence of 0.1 ng/mL recombinant mouse TNFα.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Andere Bezeichnung: TNFR1/TNFRSF1A (TNFRSF1A Produkte)

Synonyme: CD120a Protein, FPF Protein, MS5 Protein, TBP1 Protein, TNF-R Protein, TNF-R-I Protein, TNF-R55 Protein, TNFAR Protein, TNFR1 Protein, TNFR1-d2 Protein, TNFR55 Protein, TNFR60 Protein, p55 Protein, p55-R Protein, p60 Protein, TNF-R1 Protein, TNF-alphaR1 Protein, TNFRI Protein, TNFRp55 Protein, TNFalpha-R1 Protein, Tnfr-2 Protein, Tnfr1 Protein, fpf Protein, tbp1 Protein, p55-r Protein, tnf-r Protein, tnfar Protein, tnfr1 Protein, cd120a Protein, tnfr55 Protein, tnfr60 Protein, xtnfr1 Protein, wu:fj65b10 Protein, zgc:85914 Protein, TNFR I Protein, TNFRSF1A Protein, TNFA Protein, cTNF Protein, TNF receptor superfamily member 1A Protein, tumor necrosis factor receptor superfamily, member 1a Protein, TNF receptor superfamily member 1A S homeolog Protein, tumor necrosis factor Protein, TNFRSF1A Protein, Tnfrsf1a Protein, tnfrsf1a.S Protein, tnfrsf1a Protein, TNF Protein

Hintergrund:

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

Synonym: CD120a;FPF;p55;p55-R;TNF-alphaR1;TNF-R;TNF-R-I;TNF-R1;TNF-R55;TNFalpha-R1;TNFAR;Tnfr-2;Tnfr1;TNFR60;TNFRI;TNFRp55

Molekulargewicht: 21.8 kDa

NCBI Accession: NP_035739

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway