TNFRSF1A Protein (AA 30-211) (Fc Tag)

Produktnummer ABIN7275764

Produktdetails

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 30-211

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFRSF1A Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human TNFR1/CD120a/TNFRSF1A Protein

Sequenz: Leu30-Thr211

Produktmerkmale: Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu30-Thr211.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TNF alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 22.1ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Andere Bezeichnung: TNFR1 (TNFRSF1A Produkte)

Hintergrund: Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.

Molekulargewicht: 48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway