Cyclin A1 Protein (CCNA1) (His tag)

Produktnummer ABIN7317696

Produktdetails

Target: Cyclin A1 (CCNA1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses Cyclin A1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human CCNA1/Cyclin-A1 Protein (His Tag)(Active)

Sequenz: Met 1-Gln 465

Produktmerkmale: A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.

Reinheit: > 96 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Cyclin A1 (CCNA1)

Andere Bezeichnung: CCNA1/Cyclin-A1 (CCNA1 Produkte)

Synonyme: fd14f08 Protein, wu:fd14f08 Protein, wu:fi20d12 Protein, zgc:111869 Protein, MGC81965 Protein, cyclin-A1 Protein, CCNA1 Protein, MGC132235 Protein, cyclin-A1-2 Protein, Cyclin-A1 Protein, ccna1 Protein, cyclin A1 Protein, cyclin A1 L homeolog Protein, CYCLIN A1;2 Protein, cyclin A1 S homeolog Protein, CCNA1 Protein, ccna1 Protein, ccna1.L Protein, CYCA1;2 Protein, Ccna1 Protein, ccna1.S Protein

Hintergrund:

Background: Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.

Synonym: CT146

Molekulargewicht: 54.6 kDa

NCBI Accession: NP_003905

Pathways: Apoptose, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA