anti-Human LAT2 Antikörper für Immunoprecipitation

Recommended LAT2 Antibody

Antigen
Linker For Activation of T Cells Family, Member 2 (LAT2) Antikörper
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • LAT2
  • MGC139435
  • Ntal
  • Wbscr5
  • AW125574
  • Wbscr15
  • linker for activation of T-cells family member 2
  • linker for activation of T cells family, member 2
  • LAT2
  • Lat2
Epitop
AA 91-243, Cytoplasmic Domain
15
14
11
11
11
10
9
8
8
6
5
5
5
5
2
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus
123
42
31
3
3
3
1
1
1
1
1
Wirt
Maus
109
28
14
Klonalität
Monoklonal
Konjugat
Dieser LAT2 Antikörper ist unkonjugiert
8
8
6
3
3
3
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
132
43
31
29
16
12
11
6
5
3
3
2
1
Optionen

Bilder

Produktnummer ABIN94439
$ 428.84
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
21.093636 ABIN94440 FACS ICC IP WB PE Mouse IgG1 AA 91-243, Cytoplasmic Domain NAP-07 4
18.093636 ABIN94442 IHC (p) IP WB Mouse IgG2a AA 196-212 NAP-03 2
16 ABIN2937601 IF/ICC IHC IP WB Rabbit AA 30-209 Polyclonal 0
7 ABIN239823 Inhibition IHC IHC (p) IP WB Mouse IgG2a AA 196-212 NAP-03 0
5.5 ABIN120727 FACS IF IP WB Mouse IgG1 AA 91-244 NAP-07 4
5.5 ABIN536095 FACS IF IP WB Mouse IgG1 NAP-07 3
4 ABIN2469996 IP IHC WB Mouse IgG2a AA 196-212 NAP-03 0
4 ABIN1723375 IHC (p) IP WB Mouse IgG2a AA 196-212 NAP-03 0
4 ABIN5582404 IHC (p) IP WB Mouse IgG2a NAP-03 0
4 ABIN532824 FACS IF IP WB Mouse IgG1 NAP-07 0
1 ABIN207049 Inhibition FACS ICC IP WB Mouse IgG1 AA 91-243 NAP-07 0
1 ABIN596835 IP WB Mouse IgG1 AA 196-212 NAP-03 0
1 ABIN1837743 IP WB Mouse IgG1 AA 91-244 NAP-07 0
1 ABIN1837742 IP WB Mouse IgG1 AA 196-212 NAP-03 0
1 ABIN120728 IHC (p) IP WB Mouse IgG1 AA 196-212 NAP-03 1
1 ABIN2297671 IP WB Rabbit IgG N-Term Polyclonal 0
1 ABIN2314705 IP WB Mouse IgG1 AA 196-212 NAP-03 0
1 ABIN2314698 IP WB Mouse IgG1 AA 91-244 NAP-07 0
1 ABIN2314701 IC FACS IP IHC WB Mouse IgG1 AA 91-244 6D620 0
1 ABIN2314711 IP IHC WB Mouse IgG2a AA 196-212 NAP-03 0

General

Antigen Linker For Activation of T Cells Family, Member 2 (LAT2) Antikörper
Epitop AA 91-243, Cytoplasmic Domain
(15), (14), (11), (11), (11), (10), (9), (8), (8), (6), (5), (5), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(123), (42), (31), (3), (3), (3), (1), (1), (1), (1), (1)
Wirt Maus
(109), (28), (14)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser LAT2 Antikörper ist unkonjugiert
(8), (8), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(132), (43), (31), (29), (16), (12), (11), (6), (5), (3), (3), (2), (1)
Pubmed 6 Publikationen vorhanden
Hersteller

Produktdetails anti-LAT2 Antikörper

Target Details LAT2 Anwendungsinformationen Handhabung Referenzen für anti-LAT2 Antikörper (ABIN94439) Bilder
Spezifität The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Kreuzreaktivität (Details) Mouse, Human, Other not tested
Reinigung Purified by protein-A affinity chromatography
Reinheit > 95 % (by SDS-PAGE)
Immunogen Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Klon NAP-07
Isotyp IgG1
Plasmids, Primers & others

Target Details LAT2

Produktdetails anti-LAT2 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-LAT2 Antikörper (ABIN94439) Bilder zurück nach oben
Antigen
Andere Bezeichnung NTAL / LAB (LAT2 Antibody Abstract)
Hintergrund NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Pathways Fc-epsilon Rezeptor Signalübertragung

Anwendungsinformationen

Produktdetails anti-LAT2 Antikörper Target Details LAT2 Handhabung Referenzen für anti-LAT2 Antikörper (ABIN94439) Bilder zurück nach oben
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-LAT2 Antikörper Target Details LAT2 Anwendungsinformationen Referenzen für anti-LAT2 Antikörper (ABIN94439) Bilder zurück nach oben
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Referenzen für anti-LAT2 Antikörper (ABIN94439)

Produktdetails anti-LAT2 Antikörper Target Details LAT2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Duque-Afonso, Yalcin, Berg, Abdelkarim, Heidenreich, Lübbert: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, 2011

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, 2004

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002

Allgemeine Veröffentlichungen

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Bilder

Produktdetails anti-LAT2 Antikörper Target Details LAT2 Anwendungsinformationen Handhabung Referenzen für anti-LAT2 Antikörper (ABIN94439) zurück nach oben
Bilder des Herstellers
Confocal Microscopy (CM) image for anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (ABIN94439) Confocal microscopy Subcellular localization of NTAL by confocal microscopy in THP-1 ...