MDM2 Antikörper (pSer166)

Produktnummer ABIN7138447

Produktdetails anti-MDM2 Antikörper

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Bindungsspezifität: pSer166

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Kreuzreaktivität: Human

Aufreinigung: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 166 (A-I-S(p)-E-T) derived from Human MDM2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: MDM2 (MDM2 Produkte)

Synonyme: ACTFS antikoerper, HDMX antikoerper, hdm2 antikoerper, zgc:109834 antikoerper, 1700007J15Rik antikoerper, AA415488 antikoerper, Mdm-2 antikoerper, MDM2 proto-oncogene antikoerper, MDM2 proto-oncogene S homeolog antikoerper, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antikoerper, MDM2 oncogene, E3 ubiquitin protein ligase antikoerper, transformed mouse 3T3 cell double minute 2 antikoerper, MDM2 antikoerper, mdm2.S antikoerper, Mdm2 antikoerper, mdm2 antikoerper

Hintergrund:

Background: This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.

Aliases: ACTFS antibody, Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, Hdm2 antibody, HDMX antibody, MDM 2 antibody, MDM2 antibody, MDM2 oncogene E3 ubiquitin protein ligase antibody, Mdm2 p53 E3 ubiquitin protein ligase homolog antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104 kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody

UniProt: Q00987

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway