Caspase 8 Antikörper

Produktnummer ABIN6260484

Produktdetails anti-Caspase 8 Antikörper

Target: Caspase 8 (CASP8)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Spezifität: Caspase 8 Antibody detects endogenous levels of total Caspase 8

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Caspase 8

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Referenzen für anti-Caspase 8 Antikörper (ABIN6260484)

Yi, Cheng, Li, Zhao, Qu, Wang, Wang: "Protective mechanisms of purified polyphenols from pinecones of Pinus koraiensis on spleen tissues in tumor-bearing S180 mice in vivo." in: Food & function, Vol. 8, Issue 1, pp. 151-166, (2018) (PubMed).

Liu, Tang, Liu, Zhang, He, Li, Gao, Tang, Jin, Kong: "Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance." in: Cell death & disease, Vol. 9, Issue 4, pp. 419, (2018) (PubMed).

Ren, Li, Xia, Guo, Zhai, Jin, Che, Gao, Duan, Ma, Huang, Huang, Lei: "Arginine inhibits the malignant transformation induced by interferon-gamma through the NF-κB-GCN2/eIF2α signaling pathway in mammary epithelial cells in vitro and in vivo." in: Experimental cell research, Vol. 368, Issue 2, pp. 236-247, (2018) (PubMed).

Gu, Shen, Li, Bao, Lu: "Oxymatrine Causes Hepatotoxicity by Promoting the Phosphorylation of JNK and Induction of Endoplasmic Reticulum Stress Mediated by ROS in LO2 Cells." in: Molecules and cells, Vol. 41, Issue 5, pp. 401-412, (2018) (PubMed).

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: Caspase 8 (CASP8 Produkte)

Synonyme: casp8-A antikoerper, xCaspase-8 antikoerper, casp8 antikoerper, CASP8 antikoerper, CASP-8 antikoerper, FLICE antikoerper, MACH antikoerper, Mch5 antikoerper, ALPS2B antikoerper, CAP4 antikoerper, Casp-8 antikoerper, MCH5 antikoerper, caspase-8 antikoerper, zgc:92075 antikoerper, caspase 8 L homeolog antikoerper, death related ced-3/Nedd2-like protein antikoerper, caspase 8 antikoerper, caspase-8 antikoerper, caspase-8-like antikoerper, caspase 8, apoptosis-related cysteine peptidase antikoerper, casp8.L antikoerper, LOC661095 antikoerper, CASP8 antikoerper, LOC5563818 antikoerper, casp8 antikoerper, LOC100222284 antikoerper, Casp8 antikoerper

Hintergrund:

Description: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Gene: CASP8

Molekulargewicht: 55kDa

Gen-ID: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades