Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Verwendungszweck
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human GSK3A.
Spezifität
GSK3A (phospho S21) polyclonal antibody detects endogenous level of GSK3A only when phosphorylated at serine 21 and does not detect GSK3B when phosphorylated at serine 9.
Kreuzreaktivität
Human, Maus, Kaninchen
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human GSK3A.
GSK3a
Reaktivität: Human
WB, ELISA, IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Immunofluorescence (1:100-1:200) Immunohistochemistry (1:50-1:100) Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.