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SMAD4 Antikörper (C-Term)

Name: SMAD4 Antikörper (C-Term)
SKU: ABIN5537061
Price: 694.38 EUR
Availability: InStock

SMAD4 Reaktivität: Human WB, IF, IHC (p), FACS Wirt: Kaninchen Polyclonal unconjugated

Western blot analysis of SMAD4 using rabbit polyclonal SMAD4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2). (SMAD4 Antikörper (C-Term))

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SMAD4 Antikörper (C-Term))

IHC

Fluorescent confocal image of Hela cell stained with SMAD4 Antibody . (SMAD4 Antikörper (C-Term))

3 Abbildungen

Produktnummer ABIN5537061

Datenblatt (PDF)

Target Alle SMAD4 Antikörper anzeigen

SMAD4 (SMAD Family Member 4 (SMAD4))
Bindungsspezifität
11

10

9

8

7

7

7

5

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 400-428, C-Term
Reaktivität
99

42

37

4

3

3

2

2

2

2

2

2

1

1
Human
Wirt
88

11

1
Kaninchen
Passende Sekundärantikörper
Klonalität
76

24
Polyklonal
Konjugat
56

9

8

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Dieser SMAD4 Antikörper ist unkonjugiert
Applikation
65

46

36

29

22

11

9

7

6

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Aufreinigung

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Immunogen

This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4.

Isotyp

Ig Fraction

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anti-SMAD Family Member 4 (SMAD4) (AA 118-240) antibody
SMAD4 Reaktivität: Human WB, IF, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

2 mg/mL

Buffer

Supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target

SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung

SMAD4 (SMAD4 Produkte)

Synonyme

smad4 antikoerper, DPC4 antikoerper, JIP antikoerper, MADH4 antikoerper, MYHRS antikoerper, AW743858 antikoerper, D18Wsu70e antikoerper, Madh4 antikoerper, XSmad4alpha antikoerper, Xsmad4 antikoerper, dpc4 antikoerper, jip antikoerper, madh4 antikoerper, xsmad4a antikoerper, SMAD family member 4 antikoerper, Smad4 protein antikoerper, SMAD family member 4, gene 1 L homeolog antikoerper, SMAD4 antikoerper, smad4 antikoerper, Smad4 antikoerper, smad4.1.L antikoerper

Hintergrund

SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.

Molekulargewicht

60 kDa

Gen-ID

4089

UniProt

Q13485

Pathways

Zellzyklus, Chromatin Binding, Autophagie