Western Blot (1:500-1:1000) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS, 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Le, Hittelman, Liu, McWatters, Li, Mills, Bast: "Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis." in: Oncogene, Vol. 22, Issue 4, pp. 484-97, (2003) (PubMed).
Krause, Bertrand, Hue: "Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes." in: European journal of biochemistry / FEBS, Vol. 269, Issue 15, pp. 3751-9, (2002) (PubMed).
Eguchi, Iwasaki, Ueno, Frank, Motley, Eguchi, Marumo, Hirata, Inagami et al.: "Intracellular signaling of angiotensin II-induced p70 S6 kinase phosphorylation at Ser(411) in vascular smooth muscle cells. Possible requirement of epidermal growth factor receptor, Ras, ..." in: The Journal of biological chemistry, Vol. 274, Issue 52, pp. 36843-51, (2000) (PubMed).
Target
RPS6KB1
(Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))