MDM2 Antikörper (pSer185)

Produktnummer ABIN538477

Produktdetails anti-MDM2 Antikörper

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Bindungsspezifität: pSer185

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Verwendungszweck: Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2.

Spezifität: Reactivity occurs against Mouse MDM2 pS185 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot.

Kreuzreaktivität: Maus

Produktmerkmale: Antibody Reactive Against Synthetic Peptide.

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding S185 of mouse Mdm2.

Anwendungsinformationen

Applikationshinweise: ELISA (1:3000-1:12000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Referenzen für anti-MDM2 Antikörper (ABIN538477)

Stros, Muselíková-Polanská, Pospísilová, Strauss: "High-affinity binding of tumor-suppressor protein p53 and HMGB1 to hemicatenated DNA loops." in: Biochemistry, Vol. 43, Issue 22, pp. 7215-25, (2004) (PubMed).

Aslanian, Iaquinta, Verona, Lees: "Repression of the Arf tumor suppressor by E2F3 is required for normal cell cycle kinetics." in: Genes & development, Vol. 18, Issue 12, pp. 1413-22, (2004) (PubMed).

Pospísilová, Siligan, Ban, Jug, Kovar: "Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y." in: Molecular cancer research : MCR, Vol. 2, Issue 5, pp. 296-304, (2004) (PubMed).

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: MDM2 (MDM2 Produkte)

Synonyme: ACTFS antikoerper, HDMX antikoerper, hdm2 antikoerper, zgc:109834 antikoerper, 1700007J15Rik antikoerper, AA415488 antikoerper, Mdm-2 antikoerper, MDM2 proto-oncogene antikoerper, MDM2 proto-oncogene S homeolog antikoerper, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antikoerper, MDM2 oncogene, E3 ubiquitin protein ligase antikoerper, transformed mouse 3T3 cell double minute 2 antikoerper, MDM2 antikoerper, mdm2.S antikoerper, Mdm2 antikoerper, mdm2 antikoerper

Gen-ID: 17246

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway