IL12 Antikörper

Produktnummer ABIN465143

Produktdetails anti-IL12 Antikörper

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human IL-12

Aufreinigung: Protein A purified

Immunogen: CHO derived recombinant Human IL-12.

Type of Immunogen: Cell extract

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (2 - 4 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 μg/mL for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 μg/mL. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL12 / IL-12 (IL12 Produkte)

Synonyme: CLMF antikoerper, CLMF2 antikoerper, IL-12B antikoerper, NKSF antikoerper, NKSF2 antikoerper, p40 antikoerper, Il-12b antikoerper, Il12p40 antikoerper, Il-12p40 antikoerper, Il12 antikoerper, IL-12p40 antikoerper, IL-12 antikoerper, LOC100217488 antikoerper, IL-12p35 antikoerper, Il-12a antikoerper, Ll12a antikoerper, p35 antikoerper, IL12p40 antikoerper, IL12B antikoerper, IL-12 p35 antikoerper, interleukin 12B antikoerper, interleukin 12A antikoerper, interleukin 12b antikoerper, interleukin 12a antikoerper, Interleukin 12 level antikoerper, IL12B antikoerper, IL12A antikoerper, Il12b antikoerper, Il12a antikoerper, IL12 antikoerper, il12a antikoerper

Hintergrund: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome