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CD11b Antikörper

ITGAM Reaktivität: Human, Maus, Kaninchen FACS, IP, IHC (fro), IHC (p), Func Wirt: Ratte Monoclonal M1-70-15 unconjugated

Produktnummer ABIN319553

Datenblatt (PDF)

Target Alle CD11b (ITGAM) Antikörper anzeigen

CD11b (ITGAM) (Integrin alpha M (ITGAM))
Reaktivität
280

179

70

33

17

17

16

14

11

7

7

5

5

2

2

1
Human, Maus, Kaninchen
Wirt
155

114

105

4

1

1
Ratte
Passende Sekundärantikörper
Klonalität
280

96

1
Monoklonal
Konjugat
126

37

32

28

21

11

9

8

8

6

6

6

6

5

4

4

4

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD11b Antikörper ist unkonjugiert
Applikation
280

88

79

79

63

44

38

27

24

20

13

13

12

6

5

5

5

4

3

2

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Kreuzreaktivität (Details)

Species reactivity (expected):Human, Rabbit.
Species reactivity (tested):Mouse.

Aufreinigung

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin-Niveau

Low Endotoxin

Immunogen

T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.

Klon

M1-70-15

Isotyp

IgG2b

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ITGAM Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-174 FITC

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ITGAM Reaktivität: Human IHC, WB, StM Wirt: Maus Monoclonal ITGAM-3338 unconjugated

Applikationshinweise

Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Immunoprecipitation. Flow Cytometry: 1/50 - 1/100 (routinely tested in flow cytometry on mouse peritoneal macrophages). Functional Assays: Clone M1/70.15 has been reported to block iC3b binding to its receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level < 0.01EU/ug

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1.0 mg/mL

Handhabung

Avoid repeated freezing and thawing.

Lagerung

4 °C/-20 °C

Informationen zur Lagerung

Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target

CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung

CD11b (ITGAM Produkte)

Synonyme

CD11b/CD18 antikoerper, CR3 antikoerper, CR3A antikoerper, Cd11b antikoerper, F730045J24Rik antikoerper, Ly-40 antikoerper, MAC1 antikoerper, Mac-1 antikoerper, Mac-1a antikoerper, CD11B antikoerper, MAC-1 antikoerper, MAC1A antikoerper, MO1A antikoerper, SLEB6 antikoerper, C3bi antikoerper, integrin subunit alpha M antikoerper, integrin alpha M antikoerper, integrin, alpha M (complement component 3 receptor 3 subunit) antikoerper, integrin alpha-M antikoerper, ITGAM antikoerper, Itgam antikoerper, LOC100351865 antikoerper

Hintergrund

The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor

Gen-ID

10090

UniProt

P05555

Pathways

Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation