NFKBIA Antikörper (Center)

Produktnummer ABIN2856763

Produktdetails anti-NFKBIA Antikörper

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Bindungsspezifität: Center

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFKBIA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Kreuzreaktivität: Rhesusaffen

Kreuzreaktivität (Details): Rhesus Monkey (100 %)

Produktmerkmale: Rabbit Polyclonal antibody to IKB alpha (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)
IKB alpha antibody

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human IKB alpha. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:1000-1:10000*

Kommentare:

Positive Control: HepG2 , BCL-1 , C2C12 , *MCF7 , *RAW264.7, *B16F10, *HT29

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Referenzen für anti-NFKBIA Antikörper (ABIN2856763)

Hsiao, Liu, Wang, Lin, Liu: "The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome." in: Journal of biomedical science, Vol. 26, Issue 1, pp. 74, (2020) (PubMed).

Details zu NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Andere Bezeichnung: IKB alpha (NFKBIA Produkte)

Synonyme: IKBA antikoerper, MAD-3 antikoerper, NFKBI antikoerper, AI462015 antikoerper, Nfkbi antikoerper, RL/IF-1 antikoerper, ECI-6/IKBA antikoerper, NFKBIA antikoerper, ikbaB antikoerper, wu:fb81f08 antikoerper, zgc:56666 antikoerper, zgc:77616 antikoerper, ikbaA antikoerper, wu:fj16a07 antikoerper, zgc:85769 antikoerper, NFKB inhibitor alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antikoerper, NFKBIA antikoerper, Nfkbia antikoerper, nfkbiab antikoerper, nfkbiaa antikoerper

Hintergrund: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]

Cellular Localization: Cytoplasm , Nucleus

Molekulargewicht: 36 kDa

Gen-ID: 4792

Pathways: NF-kappaB Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling