CD11c Antikörper (APC)

Produktnummer ABIN2742268

Produktdetails anti-CD11c Antikörper (APC)

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD11c Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Spezifität: Integrin alpha X subunit (CD11c) of about 150 kd.

Aufreinigung: Protein A purified

Immunogen: Human rheumatoid synovial fluid cells/monocytes.

Type of Immunogen: Cells

Klon: 3-9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo

Usage: Five x 105 ficoll prepared human PBMC were washed and pre incubated 10 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD11c/APC at a 1:50 dilution (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. An 11 % sub population of the cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti- CD11c antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze. Protect from light.

Details zu CD11c

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Andere Bezeichnung: ITGAX / CD11c (ITGAX Produkte)

Synonyme: CD11C antikoerper, SLEB6 antikoerper, RGD1561123 antikoerper, AI449405 antikoerper, Cd11c antikoerper, Cr4 antikoerper, N418 antikoerper, integrin subunit alpha X antikoerper, integrin alpha X antikoerper, ITGAX antikoerper, Itgax antikoerper

Hintergrund: Name/Gene ID: ITGAX
Family: Integrin

Synonyms: ITGAX, CD11c antigen, Integrin alpha-X, Leu M5, alpha subunit, p150 95 integrin alpha chain, SLEB6, CD11C, Leu M5

Gen-ID: 3687

Pathways: Komplementsystem, Activated T Cell Proliferation, Integrin Complex