Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) Antikörper (PE)

Details zu Produkt Nr. ABIN302039
Antigen
  • CD11C
  • SLEB6
  • RGD1561123
  • AI449405
  • Cd11c
  • Cr4
  • N418
  • integrin subunit alpha X
  • integrin alpha X
  • ITGAX
  • Itgax
Reaktivität
Human, Affe
368
124
29
19
18
10
2
2
2
1
Wirt
Maus
275
106
53
37
7
3
Klonalität (Klon)
Monoklonal ()
Konjugat
PE
45
45
41
25
14
10
10
7
7
6
6
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
327
123
93
82
66
56
50
37
10
6
5
4
4
4
3
2
1
1
1
1
1
1
Optionen
Immunogen Dendritic cells of synovial fluid
Klon BU15
Isotyp IgG1
Spezifität The antibody BU15 reacts with CD11c (alphaX, p150), a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.
HLDA III, WS Code M 256
HLDA V, WS Code AS S143
HLDA VI, WS Code AS Ref.6
Kreuzreaktivität (Details) Human, Monkey
Antigen
Andere Bezeichnung CD11c (ITGAX Antibody Abstract)
Hintergrund CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.
Pathways Activated T Cell Proliferation, Integrin Complex
Applikations-hinweise The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Kommentare

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX) antibody (PE) (ABIN302039) Surface staining of human peripheral blood cells with anti-CD11c (BU15) PerCP (monocy...
Allgemeine Veröffentlichungen Bullard, Hu, Adams, Schoeb, Barnum: "p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis." in: The American journal of pathology, Vol. 170, Issue 6, pp. 2001-8, 2007 (PubMed).

Sadhu, Ting, Lipsky, Hensley, Garcia-Martinez, Simon, Staunton: "CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity." in: Journal of leukocyte biology, Vol. 81, Issue 6, pp. 1395-403, 2007 (PubMed).

Gang, Choi, Lee, Nham: "Identification of critical residues for plasminogen binding by the alphaX I-domain of the beta2 integrin, alphaXbeta2." in: Molecules and cells, Vol. 24, Issue 2, pp. 240-6, 2007 (PubMed).

Vorup-Jensen, Chi, Gjelstrup, Jensen, Jewett, Xie, Shimaoka, Linhardt, Springer: "Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin." in: The Journal of biological chemistry, Vol. 282, Issue 42, pp. 30869-77, 2007 (PubMed).

Produkt verwendet in: Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Sadhu, Hendrickson, Dick, Potter, Staunton: "Novel tools for functional analysis of CD11c: activation-specific, activation-independent, and activating antibodies." in: Journal of immunoassay & immunochemistry, Vol. 29, Issue 1, pp. 42-57, 2007 (PubMed).

Myou, Zhu, Boetticher, Qin, Myo, Meliton, Lambertino, Munoz, Hamann, Leff: "Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2." in: Immunology, Vol. 107, Issue 1, pp. 77-85, 2002 (PubMed).

Van der Vieren, Le Trong, Wood, Moore, St John, Staunton, Gallatin: "A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3." in: Immunity, Vol. 3, Issue 6, pp. 683-90, 1996 (PubMed).

Hogg, Takacs, Palmer, Selvendran, Allen: "The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules." in: European journal of immunology, Vol. 16, Issue 3, pp. 240-8, 1986 (PubMed).

Haben Sie etwas anderes gesucht?