TNFRSF1A Antikörper

Produktnummer ABIN2688923

Produktdetails anti-TNFRSF1A Antikörper

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNFRSF1A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Marke: BD Pharmingen™

Produktmerkmale: The TNFR1-B1 antibody reacts with the extracellular domain of CD120A which is also known as the 55 kDa receptor for the human cytokines, tumor necrosis factor (TNF) and lymphotoxin-alpha (LT-α). This receptor is referred to as the p55 or Type I Tumor Necrosis Factor Receptor (TNFRI). TNFRI are expressed by a variety of cell lines, tumor cells, and normal cell types including T cells, monocytes, macrophages, neutrophils, endothelial cells, hepatocytes, chondrocytes, and fibroblasts. Naive B cells express very low or undetectable levels of TNFRI whereas mature erythrocytes and platelets are uniformly negative for TNFRI expression. For the generation of the MABTNFR1-B1 hybridoma, BALB/c mice were immunized with purified, full-length human TNFRI protein that was expressed by insect cells that were infected with a recombinant human TNFRI-baculovirus expression vector. MABTNFR1-B1 specifically binds to natural and recombinant truncated forms of TNFRI. Expression of cell surface TNFRI by non-activated human PBMC or RBC. Human PBMC (left panel) and red blood cells (right panel) isolated by density centrifugation (Ficoll-Paque™) were stained with MABTNFR1-B1 (0.5 μg, Cat No. 550514) followed by biotinylated goat anti-mouse Ig (0.25 μg, Cat . No. 553999) and Streptavidin Phycoerythrin (0.015 μg, Cat. No. 554061) in a three-step staining protocol to amplify immunofluorescent signals. Staining with the MABTNFR1-B1 antibody (filled histograms) is compared to staining obtained using the secondary antibody alone (open histograms). Histograms in the left panel are gated on the lymphocyte population. Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.

BD Pharmingen™ Purified Mouse Anti-Human CD120a - Purified - Clone MABTNFR1-B1 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.5 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Klon: MABTNFR1

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-TNFRSF1A Antikörper (ABIN2688923)

Browning, Dougas, Ngam-ek, Bourdon, Ehrenfels, Miatkowski, Zafari, Yampaglia, Lawton, Meier: "Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 33-46, (1995) (PubMed).

Wallach, Engelmann, Nophar, Aderka, Kemper, Hornik, Holtmann, Brakebusch: "Soluble and cell surface receptors for tumor necrosis factor." in: Agents and actions. Supplements, Vol. 35, pp. 51-7, (1992) (PubMed).

Heilig, Mapara, Brockhaus, Krauth, Dörken: "Two types of TNF receptors are expressed on human normal and malignant B lymphocytes." in: Clinical immunology and immunopathology, Vol. 61, Issue 2 Pt 1, pp. 260-7, (1991) (PubMed).

Hohmann, Remy, Brockhaus, van Loon: "Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha)." in: The Journal of biological chemistry, Vol. 264, Issue 25, pp. 14927-34, (1989) (PubMed).

Munker, DiPersio, Koeffler: "Tumor necrosis factor: receptors on hematopoietic cells." in: Blood, Vol. 70, Issue 6, pp. 1730-4, (1988) (PubMed).

Details zu TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Andere Bezeichnung: CD120a (TNFRSF1A Produkte)

Synonyme: CD120a antikoerper, FPF antikoerper, MS5 antikoerper, TBP1 antikoerper, TNF-R antikoerper, TNF-R-I antikoerper, TNF-R55 antikoerper, TNFAR antikoerper, TNFR1 antikoerper, TNFR1-d2 antikoerper, TNFR55 antikoerper, TNFR60 antikoerper, p55 antikoerper, p55-R antikoerper, p60 antikoerper, TNF-R1 antikoerper, TNF-alphaR1 antikoerper, TNFRI antikoerper, TNFRp55 antikoerper, TNFalpha-R1 antikoerper, Tnfr-2 antikoerper, Tnfr1 antikoerper, fpf antikoerper, tbp1 antikoerper, p55-r antikoerper, tnf-r antikoerper, tnfar antikoerper, tnfr1 antikoerper, cd120a antikoerper, tnfr55 antikoerper, tnfr60 antikoerper, xtnfr1 antikoerper, wu:fj65b10 antikoerper, zgc:85914 antikoerper, TNFR I antikoerper, TNFRSF1A antikoerper, TNFA antikoerper, cTNF antikoerper, TNF receptor superfamily member 1A antikoerper, tumor necrosis factor receptor superfamily, member 1a antikoerper, TNF receptor superfamily member 1A S homeolog antikoerper, tumor necrosis factor antikoerper, TNFRSF1A antikoerper, Tnfrsf1a antikoerper, tnfrsf1a.S antikoerper, tnfrsf1a antikoerper, TNF antikoerper

Hintergrund: Synonyms: TNF Receptor Type I

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Hepatitis C, Ubiquitin Proteasome Pathway