AKT2 Antikörper (C-Term) (Biotin)

Produktnummer ABIN2486860

Produktdetails anti-AKT2 Antikörper (Biotin)

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~65 kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).

Kreuzreaktivität: Huhn, Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf, Xenopus laevis

Aufreinigung: Peptide Affinity Purified

Immunogen: A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• ICC/IF (1:25)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:3000 dilution of ABIN2486860 was sufficient for detection of PKBβ in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: TBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Andere Bezeichnung: AKT2 (AKT2 Produkte)

Synonyme: AKT2 antikoerper, pkbb antikoerper, pkbbeta antikoerper, pkbss antikoerper, prkbb antikoerper, rac-beta antikoerper, v-akt2 antikoerper, 2410016A19Rik antikoerper, AW554154 antikoerper, PKB antikoerper, PKBbeta antikoerper, HIHGHH antikoerper, PKBB antikoerper, PKBBETA antikoerper, PRKBB antikoerper, RAC-BETA antikoerper, cb945 antikoerper, akt2-a antikoerper, AKT serine/threonine kinase 2 antikoerper, v-akt murine thymoma viral oncogene homolog 2 antikoerper, thymoma viral proto-oncogene 2 antikoerper, v-akt murine thymoma viral oncogene homolog 2 L homeolog antikoerper, AKT2 antikoerper, akt2 antikoerper, Akt2 antikoerper, akt2.L antikoerper

Hintergrund: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).

Gen-ID: 208

NCBI Accession: NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling