AKT2 Antikörper

Produktnummer ABIN1892264

Produktdetails anti-AKT2 Antikörper

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser AKT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88 % identity to mouse and 90 % identity with rat, therefore, cross reactivity is expected.

Aufreinigung: Protein A purified

Sterilität: Sterile filtered

Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Type of Immunogen: Synthetic peptide

Klon: 11F6-B2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:2000 - 1:10000), IHC (20 μg/mL), WB (1:500 - 1:2000)

Usage: Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, sterile filtered

Konservierungsmittel: Azide free

Handhabung: avoid freeze thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Details zu AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Andere Bezeichnung: AKT2 (AKT2 Produkte)

Synonyme: AKT2 antikoerper, pkbb antikoerper, pkbbeta antikoerper, pkbss antikoerper, prkbb antikoerper, rac-beta antikoerper, v-akt2 antikoerper, 2410016A19Rik antikoerper, AW554154 antikoerper, PKB antikoerper, PKBbeta antikoerper, HIHGHH antikoerper, PKBB antikoerper, PKBBETA antikoerper, PRKBB antikoerper, RAC-BETA antikoerper, cb945 antikoerper, akt2-a antikoerper, AKT serine/threonine kinase 2 antikoerper, v-akt murine thymoma viral oncogene homolog 2 antikoerper, thymoma viral proto-oncogene 2 antikoerper, v-akt murine thymoma viral oncogene homolog 2 L homeolog antikoerper, AKT2 antikoerper, akt2 antikoerper, Akt2 antikoerper, akt2.L antikoerper

Hintergrund: Name/Gene ID: AKT2
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT2, PKB beta, PKBBETA, PRKBB, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, Rac protein kinase beta, RAC-BETA, HIHGHH, PKBB

Gen-ID: 208

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling