RFP Antikörper (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN1573924, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
81
50
37
9
7
1
1
Wirt
Kaninchen
94
37
14
9
1
Klonalität
Polyklonal
Konjugat
Dieser RFP Antikörper ist unkonjugiert
9
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
132
67
60
53
32
27
6
5
4
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated - KLH
Isotyp IgG
Spezifität Rabbit Anti-RFP-tag Polyclonal Antibody specifically reacts with fusion proteins containing RFP-tags. The antibody does not react with GFP or GFP-tagged proteins.
Kreuzreaktivität (Details) RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. The antibody does not react with GFP or GFP-tagged proteins.
Reinigung Immunoaffinity chromatography
Andere Bezeichnung RFP-Tag (RFP Antibody Abstract)
Hintergrund Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1573924) Loading: RFP-tag fusion protein expressed in E. coli cell lysate Primary antibody: 1 ...
Western Blotting (WB) image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1573924) Lane 1: MarkerLane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed ...
Produkt verwendet in: Alvarez-Guaita, Vilà de Muga, Owen, Williamson, Magenau, García-Melero, Reverter, Hoque, Cairns, Cornely, Tebar, Grewal, Gaus, Ayala-Sanmartín, Enrich, Rentero: "Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains." in: British journal of pharmacology, Vol. 172, Issue 7, pp. 1677-90, 2015 (PubMed).

Yang, Shen, Garre, Hao, Krumlinde, Cvijovi?, Arens, Nyström, Liu, Sunnerhagen: "Stress granule-defective mutants deregulate stress responsive transcripts." in: PLoS genetics, Vol. 10, Issue 11, pp. e1004763, 2014 (PubMed).

Devers, Teply, Reinert, Gaude, Krajinski: "An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula." in: BMC plant biology, Vol. 13, pp. 82, 2013 (PubMed).

Roberts, Baines: "Myosin Va enhances secretion of herpes simplex virus 1 virions and cell surface expression of viral glycoproteins." in: Journal of virology, Vol. 84, Issue 19, pp. 9889-96, 2010 (PubMed).

Nilsson, Sunnerhagen: "Cellular stress induces cytoplasmic RNA granules in fission yeast." in: RNA (New York, N.Y.), Vol. 17, Issue 1, pp. 120-33, 2010 (PubMed).

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