RFP Antikörper (Red Fluorescent Protein) (HRP)

Produktnummer ABIN129725

Produktdetails anti-RFP Antikörper

Target: RFP

Reaktivität: Discosoma

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit HRP

Applikation: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Do NOT add Sodium Azide!

Lagerung: 4 °C

Referenzen für anti-RFP Antikörper (ABIN129725)

Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

Details zu RFP

Target: RFP

Andere Bezeichnung: RFP (RFP Produkte)

Hintergrund: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: HRP, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583