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RFP Antikörper (Biotin)

RFP Reaktivität: Discosoma WB, ELISA Wirt: Huhn Polyclonal Biotin
Produktnummer ABIN1607901
  • Target Alle RFP Antikörper anzeigen
    RFP (Red Fluorescent Protein (RFP))
    Reaktivität
    • 52
    • 1
    Discosoma
    Wirt
    • 23
    • 15
    • 9
    • 5
    • 2
    Huhn
    Klonalität
    • 32
    • 22
    Polyklonal
    Konjugat
    • 21
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RFP Antikörper ist konjugiert mit Biotin
    Applikation
    • 28
    • 22
    • 14
    • 12
    • 10
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Verwendungszweck
    Anti-RFP is designed to detect recombinant RFP.
    Aufreinigung
    RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by ELISA, western blot and immunoelectrophoresis.
    Immunogen
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
    Immunogen Type: Recombinant Protein
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  • Applikationshinweise
    Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
    ELISA Dilution: 1:10.000
    Western Blot Dilution: 1:1.000 - 1:3.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Restore with 100 µL deionized water (or equivalent)
    Konzentration
    1 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." in: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

  • Target
    RFP (Red Fluorescent Protein (RFP))
    Abstract
    RFP Produkte
    Hintergrund
    Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    Gen-ID
    55976617
    UniProt
    Q9U6Y8
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