This antibody recognises the CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyteassociated antigen 4).
Aufreinigung
Affinity Chromatography
Immunogen
Human CTLA-4/human IgG heavy chain fusion protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.
Flow Cytometry: Use 20 μL of neat antibody to label 5x10e5 cells in 100 μL. Membrane permeabilisation is required for this application. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
PBS containing 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use
Lagerung
4 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Target
CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present within the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response.Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4