IL-6 Antikörper

Produktnummer ABIN116084

Der Ziege Polyklonal anti-IL-6 Antikörper wird verwendet zum Nachweis von IL-6 in Proben von Human. Er wurde validiert für WB, IHC (p), EIA und Func.

Kurzübersicht für IL-6 Antikörper (ABIN116084)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL-6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-IL-6 Antikörper

Spezifität: This antibody detects Interleukin-6.

Aufreinigung: Affinity chromatography employing immobilized Human IL-6 matrix

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human IL-6

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: This antibody stained FFPE sections of human cervical squamous cell carcinoma. Recommended dilution: 2.5 μg/mL (overnight at 4C). Use heat induced antigen retrieval with a pH 6.0 sodium citrate buffer. An HRP-labeledpolymer detection system was used with a DAB chromogen. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (5 ng/mL), aconcentration of 0.05 - 0.08 μg/mL of this antibody is required. Elisa(Indirect): To detect hIL-6 by indirect ELISA (using 100 μL/well antibody solution) a

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Haltbarkeit: 12 months

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: Interleukin-6 (IL6)

Hintergrund: Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2

Gen-ID: 3569

NCBI Accession: NP_000591

UniProt: P05231

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome