TLR9 Antikörper (Extracellular Domain, N-Term) (FITC)

Produktnummer ABIN1106226

Produktdetails anti-TLR9 Antikörper (FITC)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Bindungsspezifität: AA 1-815, Extracellular Domain, N-Term

Reaktivität: Human, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TLR9 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: The monoclonal antibody 5G5 recognizes human Toll-like receptor 9.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, canine

Aufreinigung: Protein G

Immunogen: Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc

Klon: 5G5

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 1 % bovine serum albumin, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Andere Bezeichnung: CD289 / TLR9 (TLR9 Produkte)

Synonyme: CD289 antikoerper, toll like receptor 9 antikoerper, toll-like receptor 9 antikoerper, TLR9 antikoerper, Tlr9 antikoerper

Hintergrund: Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens. Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.Synonyms: Toll-like receptor 9, UNQ5798/PRO19605

Gen-ID: 81897

NCBI Accession: NP_112455

UniProt: Q9EQU3

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades