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TLR9 Antikörper (AA 868-1016)

TLR9 Reaktivität: Human WB, IHC, ELISA, FACS, ICC Wirt: Maus Monoclonal 1B12H2 unconjugated
Produktnummer ABIN5542492
  • Target Alle TLR9 Antikörper anzeigen
    TLR9 (Toll-Like Receptor 9 (TLR9))
    Bindungsspezifität
    • 19
    • 15
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 868-1016
    Reaktivität
    • 93
    • 39
    • 13
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 64
    • 32
    • 7
    • 4
    • 1
    Maus
    Klonalität
    • 72
    • 36
    Monoklonal
    Konjugat
    • 61
    • 13
    • 12
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TLR9 Antikörper ist unkonjugiert
    Applikation
    • 76
    • 48
    • 44
    • 44
    • 23
    • 15
    • 11
    • 11
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Aufreinigung
    purified
    Immunogen
    Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. coli.
    Klon
    1B12H2
    Isotyp
    IgG2a
  • Applikationshinweise
    ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #103693 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Klinik für Anästhesie, Intensivmedizin und Schmerztherapie, Universitätsklinikum der Ruhr Universität Bochum
    No.
    #103693
    Datum
    26.03.2019
    Antigen
    TLR9
    Chargennummer
    160127
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle

    U937 monocytic cells

    Negativkontrolle

    No primary antibody control

    Bewertung

    Passed. ABIN5542492 localization on ER is visible. Negative control without primary antibody shows no signal.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN5542492
    Sekundärantikörper
    AF488-conjugated AffiniPure alpaca anti-Mouse IgG antibody (Jackson ImmunoResearch, 615-545-214)
    Full Protocol
    • Grow U937 cells in RPMI (Gibco, 21875-034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep(Gibco, 15140-122), at 37°C and 5% CO2 in 500µL in 12-well plates.
    • Use cytospin to spin cells onto slide.
    • Fix cells on coverslide in 4% PFA for 20min at RT.
    • Wash cells 3x for 5min with PBS.
    • Permeabilize cells in PBS containing 0.1% Triton for 5min at RT.
    • Block non-specific binding with Duolink PLA Blocking Solution (Sigma) for 30 min at RT.
    • Incubate cells with primary mouse anti-TLR9 antibody (antibodies-online, ABIN5542492, lot 160127) diluted 1:200 in staining solution ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-Mouse IgG antibody (Jackson ImmunoResearch, 615-545-214) diluted 1:400 in staining solution for 1h at RT.
    • Wash cells 2x for 5min with PBS.
    • Mount coverslips on glass slides in ProLong Gold antifade reagent (Invitrogen, P36935,1890418) containing DAPI.
    • Image acquisition with an Olympus widefield microscope.
    Anmerkungen

    TLR9 staining using ABIN5542492 shows typical localization peri-nuclear localization.

  • Validierung #103821 (Proximity Ligation Assay)
    'Independent Validation' Siegel
    by
    Klinik für Anästhesie, Intensivmedizin und Schmerztherapie, Universitätsklinikum der Ruhr Universität Bochum
    No.
    #103821
    Datum
    29.04.2019
    Antigen
    TLR9
    Chargennummer
    160127
    Validierte Anwendung
    Proximity Ligation Assay
    Positivkontrolle

    U937 monocytic cells stimulated with ODN1826

    Negativkontrolle

    Anti TLR9 antibody (ABIN5542492) alone

    Anti pY antibody (ABIN361758) alone

    Bewertung

    Passed. PLA signal visible in stimulated cells. Negative control with only one primary antibody shows no signal.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN5542492
    Sekundärantikörper
    ABIN361758
    Full Protocol
    • Grow U937 cells in RPMI (Gibco, 21875-034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep(Gibco, 15140-122), at 37°C and 5% CO2 in 500µL in 12-well plates.
    • Incubate the cells with 1µM ODN1826 for 4h.
    • Use cytospin to spin cells onto slide.
    • Fix cells on coverslide in 4% PFA for 20min at RT.
    • Wash cells 3x for 5min with PBS.
    • Permeabilize cells in PBS containing 0.1% Triton for 5min at RT.
    • Block non-specific binding with Duolink PLA Blocking Solution (Sigma-Aldrich) for 30min at RT.
    • Incubate cells with primary mouse anti-TLR9 antibody (antibodies-online, ABIN5542492, lot 160127) diluted 1:200 and primary rabbit anti-pY antibody (antibodies-online, ABIN361758, lot 1508290) diluted 1:100 ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with Duolink In Situ PLA Probe anti-Rabbit MINUS (Sigma-Aldrich, DUO92005, lot SLBZ4516) and Duolink In Situ PLA Probe anti-Mouse mouse PLUS (Sigma-Aldrich, DUO92001, lot SLBZ8369) proximity probes according to manufacturers recommendations.
    • Wash cells 2x for 5min with PBS.
    • Perform Ligation step according to the Duolink PLA kit (Sigma).
    • Perform Amplification step according to the Duolink In Situ PlA Detection Kit Green (Sigma-Aldrich, DUO92014).
    • Mount coverslips on glass slides in ProLong Gold antifade reagent (Invitrogen, P36935, lot 1890418) containing DAPI.
    • Image acquisition with an Olympus widefield microscope.
    Anmerkungen

    We used double the amount the Phi29 polymerase for the amplifaction step than recommended.

  • Format
    Liquid
    Buffer
    Purified antibody in PBS with 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    4°C, -20°C for long term storage
  • Target
    TLR9 (Toll-Like Receptor 9 (TLR9))
    Andere Bezeichnung
    TLR9 (TLR9 Produkte)
    Synonyme
    CD289 antikoerper, toll like receptor 9 antikoerper, toll-like receptor 9 antikoerper, TLR9 antikoerper, Tlr9 antikoerper
    Hintergrund

    Description: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.,

    Aliases: CD289

    Molekulargewicht
    115.8 kDa
    Gen-ID
    54106
    HGNC
    54106
    Pathways
    TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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