RFP Antikörper

Produktnummer ABIN1103975

Produktdetails anti-RFP Antikörper

Target: RFP (Red Fluorescent Protein (RFP))

Reaktivität: Discosoma

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser RFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: Anti-RFP / Red Fluorescent Protein Antibody

Spezifität: Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (1:1000 - 1:3000)

Usage: Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide, sterile filtered

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid freeze/thaw cycles. Store undiluted.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Store undiluted.

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP (RFP Produkte)